壳聚糖—阿托伐他汀偶联物表面改性钛材及其细胞相容性研究

发布时间：2018-06-09 15:08

本文选题：钛 + 壳聚糖-阿托伐他汀偶联物　；参考：《重庆大学》2013年硕士论文

【摘要】：钛材作为一种生物医用植入材料，被广泛地应用于临床治疗，如假肢、心血管植入物和种植牙等植入体。然而，在某些情形下，钛材植入体内几小时后会发生炎症反应，临床表现主要包括植入部位发红、发热和疼痛等症状。本文旨在以钛材为基材，通过改善其表面的相关特性，进而减少组织对钛材的炎症响应，提高钛材的生物相容性。首先对合成的壳聚糖-阿托伐他汀偶联物通过FTIR、H1NMR进行了表征，然后在钛材表面形成聚多巴胺薄膜作为中间层来固定壳聚糖-阿托伐他汀偶联物，分别用场发射扫描电镜（FE-SEM），X-射线光谱图（XPS）和接触角进行表征。实验结果表明，壳聚糖-阿托伐他汀偶联物成功固定在钛材表面。相关细胞实验表明，接种在固载壳聚糖-阿托伐他汀偶联物的钛材上的内皮细胞分别经过4天和7天的培养后较其他对照组表现出更高的细胞活性和更低的乳酸脱氢酶产物（p0.01）。同时，在固载壳聚糖-阿托伐他汀偶联物的钛材上，，RAW264.7细胞较纯钛基材表现出更低水平的一氧化氮（NO）、酸性磷酸酶（ACP）活性、活性氧（ROS）和前炎症因子包括肿瘤坏死因子（TNF-α）和白介素（IL-1β）（p0.01）。该结果表明，固载壳聚糖-阿托伐他汀偶联物的钛材能抑制巨噬细胞的炎症响应且具有更好的生物相容性。本研究为构建生物功能性钛基材植入物进一步用于生物医药领域提供了新的参考方法。
[Abstract]:Titanium, as a biomedical implant, is widely used in clinical treatment, such as prostheses, cardiovascular implants and dental implants. However, in some cases, a few hours after titanium implantation, inflammation occurs, including symptoms such as redness, fever and pain at the implant site. Furthermore, the inflammatory response of tissue to titanium is reduced, and the biocompatibility of titanium is improved. The synthesized chitosan-Atto valvastatin conjugate was characterized by FTIR H 1 NMR, and then the poly dopamine film was formed on the titanium surface as the intermediate layer to immobilize the chitosan-Atto vastatin conjugate. Field emission scanning electron microscopy (SEM) and contact angle were used to characterize the results. The results showed that chitosan-Atto vastatin conjugate was successfully immobilized on titanium surface. The related cell experiments showed that the endothelial cells inoculated with chitosan-Atto vastatin conjugate showed higher cell activity and lower lactate dehydrogenase product than those of other control groups after 4 and 7 days culture respectively. At the same time, RAW264.7 cells exhibited lower activity of nitric oxide (no), acid phosphatase (ACP) and proinflammatory factors (including tumor necrosis factor TNF- 伪) and interleukin-1 尾 (p0.01) than pure titanium substrates. The results showed that titanium supported on chitosan-Atto vastatin conjugate could inhibit the inflammatory response of macrophages and have better biocompatibility. This study provides a new reference method for the construction of biofunctional titanium substrate implants for biomedical applications.
【学位授予单位】：重庆大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R318.08

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