粉末冶金注射成型技术制备的新型血管内支架的动物应用研究

发布时间：2018-06-11 11:21

本文选题：支架 + 材料试验　；参考：《中国普通外科杂志》2017年02期

【摘要】：目的:通过动物实验评估一种新型血管内支架临床应用的可行性和安全性。方法:将30枚粉末冶金注射成型技术制备新型血管内支架分别植入30只实验犬主动脉内,术后CT血管造影了解其在主动脉内情况,并通过大体肉眼观察、光镜、电镜及免疫组化了解内术后不同时间支架表面新生内膜情况。结果:所有支架均成功植入实验犬主动脉内。术后支架通畅率100%,无支架移位、扭曲、断裂,无支架感染及血栓形成,管腔无狭窄或闭塞;支架的轴向回缩率均2%,径向回缩率均4%。术后1周,支架腔面迅速被一薄层半透明膜状结构覆盖;术后1个月,支架绝大部分表面可见与周围血管正常内膜相延续的新生内膜;术后2个月,新生内膜基本上完整覆盖整个支架腔面(98.83%),其厚度达到峰值(350.00μm);术后3~6个月,新生内膜厚度逐渐降低,管腔内径逐渐增大至植入前大小,最后新生内膜表面被单层完全成熟的内皮细胞覆盖。除术后1周外,其余时间点支架表面新生内膜组织中血管平滑肌细胞α-肌动蛋白染色均呈阳性,而各时间点支架腔面新生内膜组织中血管内皮生长因子染色均呈阳性。结论:粉末冶金注射成型技术制备的新型血管内支架植入后实验犬体内后形态结构稳定,并可迅速完成支架腔面内皮化,保持长期的通畅性,表现出良好的结构及理化稳定性和生物相容性,具有很好的临床应用前景。
[Abstract]:Objective: to evaluate the feasibility and safety of clinical application of a new type of endovascular stent through animal experiments. Methods: thirty new intravascular stents were implanted into the aorta of 30 experimental dogs by powder metallurgy injection molding technique. After operation, CT angiography was performed to find out the situation in the aorta. The neointima of stent surface at different time after operation were observed by electron microscope and immunohistochemistry. Results: all the stents were successfully implanted into the aorta of experimental dogs. The patency rate of stent was 100%, without stent displacement, distortion, rupture, no stent infection and thrombosis, no stenosis or occlusion in the lumen, the axial retraction rate was 2%, and the radial retraction rate was 4%. One week after the operation, the stent cavity was quickly covered by a thin translucent membrane structure. 1 month after operation, most of the neointima continued with the normal intima of the surrounding vessels, and 2 months after the operation, the stent was covered by a thin translucent membrane structure. The neointima covered the whole stent cavity completely, and the thickness reached the peak value of 350.00 渭 m, the thickness of neointima gradually decreased and the diameter of the lumen gradually increased to the size before implantation 3 ~ 6 months after operation. Finally, the surface of the neointima is covered by a monolayer of fully mature endothelial cells. The 伪 -actin staining of vascular smooth muscle cells was positive in the neointimal tissue of the stent at other time points except one week after operation, but the expression of vascular endothelial growth factor was positive in the neointimal tissue of the stent at each time point. Conclusion: the new intravascular stents prepared by powder metallurgy injection molding technology are stable in morphology and structure of experimental dogs after implantation, and can quickly finish the endothelialization of stents cavity and maintain the long-term patency. It shows good structure, physicochemical stability and biocompatibility, and has a good prospect of clinical application.
【作者单位】：中南大学湘雅二医院血管外科;中国医学科学院阜外医院血管外科;湘潭大学材料科学与工程学院;中南大学粉末冶金国家重点实验室;
【分类号】：R318.08

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