分层多功能层层自组装肝素表面涂层的制备和血液相容性检测

发布时间：2018-06-12 17:36

本文选题：心室辅助装置 + 表面修饰　；参考：《北京协和医学院》2013年硕士论文

【摘要】：背景：心室辅助装置在临床应用取得了良好的治疗效果,同时也面临着长期使用引起的出血、感染、血栓形成等并发症。要减少这些并发症,都亟待改进心室辅助装置与血液接触面的血液相容性。涂层技术可以在不改变基材的前提下改善材料表面性质,提高血液相容性,从而减少人工植入装置植入后的不良反应。目的：制备分层多功能层层自组装肝素涂层,并对照研究该涂层的血液相容性及抗凝特性与传统涂层的差别。方法：利用层层自组装技术组装出有底层和表层两个功能层的多层自组装涂层,底层为壳聚糖层,通过共价键固定在钛金属表面,表层为多层肝素/Fe3+层层自组装涂层,共5层肝素。以组装相同层数的肝素/壳聚糖层层自组装涂层作为多层对照组；单层对照组为肝素和壳聚糖共混溶液的单层涂层；空白对照组为裸钛片。对照研究不同涂层的肝素含量、肝素释放量,以及血液相容性的差别。结果：测定分层多功能自组装涂层的肝素总含量为：7.57±0.55μg/cm2,优于多层对照组和单层对照组的5.13±0.57μg/cm2和1.55±0.79μg/cm2(P0.05),同时实验组4,12,24,48小时肝素溶解释放量均明显高于多层对照组和单层对照组。实验制备的分层多功能涂层在溶血率测定也优于多层对照组和空白对照组,同时5%的溶血率符合国家标准。动态凝血实验显示实验制备的分层多功能自组装涂层抗凝性能最好,每个时间点抗凝血程度均优于对照组。结论：分层多功能层层自组装肝素涂层在相同的组装条件和组装层数时,能够组装更多的肝素,同时有利于肝素的溶解释放,能够提高材料的血液相容性和抗凝血性能。
[Abstract]:Background: ventricular assistive device has achieved good therapeutic effect in clinical application, and also faces complications such as bleeding, infection and thrombosis caused by long-term use. To reduce these complications, it is necessary to improve the blood compatibility of ventricular assistive devices and blood interface. The coating technology can improve the surface properties of the material without changing the substrate and improve the blood compatibility, thus reducing the adverse reactions after the implantation of the artificial implants. Aim: to prepare layered multifunctional layer self assembled heparin coating and compare the blood compatibility and anticoagulant characteristics between the coating and the traditional coating. Methods: the multilayer self-assembly coating with two functional layers was assembled by layer self-assembly technique. The bottom layer was chitosan layer, which was fixed on titanium surface by covalent bond, and the surface layer was multi-layer heparin / Fe _ 3 layer self-assembly coating. A total of 5 layers of heparin. The layers of heparin / chitosan self-assembled coatings with the same number of layers were used as the multilayer control group; the monolayer control group was the monolayer coating of heparin and chitosan blend solution; and the blank control group was a bare titanium sheet. The heparin content, heparin release and blood compatibility of different coatings were studied. Results: the total heparin content of the multifunctional self-assembled coating was 7. 57 卤0. 55 渭 g / cm ~ 2, which was better than that of the multilayer control group and the monolayer control group (5. 13 卤0. 57 渭 g/cm2 and 1. 55 卤0. 79 渭 g / cm ~ (2) P 0. 05). Meanwhile, the heparin release of the experimental group at 48 hours was significantly higher than that of the multilayer control group and the monolayer control group. The multifunctional coating was also superior to the multilayer control group and the blank control group in the determination of hemolysis rate, and the hemolysis rate of 5% was in line with the national standard. The dynamic coagulation experiment showed that the layered multifunctional self-assembled coating had the best anticoagulant performance and the anticoagulant degree at each time point was better than that of the control group. Conclusion: the layered multifunctional layer self-assembled heparin coating can assemble more heparin at the same assembly conditions and the number of the assembled layers, and it is beneficial to the dissolution and release of heparin, and can improve the blood compatibility and anticoagulant performance of the materials.
【学位授予单位】：北京协和医学院
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R318.08

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