同种异体软骨细胞-聚羟基乙酸支架复合物修复甲状软骨缺损

发布时间：2018-06-14 20:16

  本文选题：甲状软骨 + 软骨细胞　； 参考：《中国组织工程研究》2016年12期

【摘要】：背景:将聚羟基乙酸支架与软骨细胞复合,可获得组织工程人工骨。目的:观察同种异体软骨细胞-聚羟基乙酸支架复合物修复乳兔喉甲状软骨缺损的效果。方法:取新西兰成年大白兔20只,构建喉甲状软骨缺损模型后随机分组,实验组于缺损处植入同种异体软骨细胞-聚羟基乙酸支架复合物,对照组于缺损处植入聚羟基乙酸支架。植入后4,8周进行大体观察、组织学观察。结果与结论:(1)大体观察结果:植入后4周,实验组软骨缺损得到一定的修复,修复区域未出现坏死;对照组缺损区域填充有肌肉和结缔组织。植入后8周,实验组软骨缺损得到进一步修复,修复界限不明显;对照组软骨缺损未得到修复,与周围正常软骨之间存在明显界限。(2)免疫组织化学染色结果:实验组植入后4,8周的Ⅱ型胶原表达均高于对照组(P0.05)。结果表明同种异体软骨细胞-聚羟基乙酸支架复合物可促进乳兔喉甲状软骨缺损的修复。
[Abstract]:Background: tissue engineering artificial bone can be obtained by combining polyglycolic acid scaffold with chondrocytes. Aim: to observe the effect of allogeneic chondrocyte-polyglycolic acid scaffold complex in repairing laryngeal thyroid cartilage defect in milk rabbits. Methods: twenty New Zealand adult rabbits were randomly divided into two groups after the laryngeal thyroid cartilage defect model was established. The experimental group implanted allogenic chondrocyte-polyglycolic acid scaffold complex in the defect. In the control group, polyglycolic acid stent was implanted at the defect. Gross observation and histological observation were made 8 weeks after implantation. Results and conclusion: 4 weeks after implantation, the cartilage defects in the experimental group were repaired to a certain extent, and no necrosis was found in the repaired region, while the defect area in the control group was filled with muscle and connective tissue. 8 weeks after implantation, the cartilage defects in the experimental group were further repaired, the repair limits were not obvious, and the cartilage defects in the control group were not repaired. The results of immunohistochemical staining showed that the expression of type 鈪，

本文编号：2018826