类风湿因子免疫吸附血液灌流材料的制备及其性能评价

发布时间：2018-06-15 21:31

本文选题：类风湿因子 + 免疫吸附剂　；参考：《中国生物医学工程学报》2014年03期

【摘要】：制备一种新型的类风湿因子(RF)免疫吸附剂并研究其性能。通过在氯甲基聚苯乙烯-二乙烯树脂(氯球)的表面接枝苯丙氨酸(PHE),制备出可供临床应用的类风湿因子血液灌流吸附剂(PS-PHE);体外动态灌流实验测定吸附剂的吸附率;体外动态洗脱实验测定RF的脱落量及脱落率;通过体外灌流模拟实验,检测灌流对血液成分的影响来评价吸附剂的选择性;体外凝血酶原时间(PTs)及凝血酶时间(TTs)检测实验验证材料的血液相容性。免疫吸附剂对类风湿因子IgA RF、IgG RF、IgM RF的吸附率分别可以达到45.21%±1.80%、56.02%±1.36%、52.40%±2.01%(n=5),洗脱后脱落率分别为22.10%±1.65%、19.23%±1.06%、21.31%±1.35%(n=5)。全血灌流实验中材料对红细胞、血小板、总蛋白的吸附均在10%以下。同时体外凝血酶原时间(PTs)及凝血酶时间(TTs)测定结果显示PS-PHE能延缓凝血速度。结论 PS-PHE对RF具有较高的吸附率及特异性并且表现出优良的血液相容性,在类风湿关节炎的临床治疗方面具有良好的应用前景。
[Abstract]:A new type of rheumatoid factor (RF) immune adsorbent was prepared and its performance was studied. By grafting phenylalanine (PHE) on the surface of chloromethyl polystyrene two vinyl resin (chlorosphere), a clinical application of rheumatoid factor hemoperfusion adsorbent (PS-PHE) was prepared. In vitro dynamic perfusion test was used to determine adsorption rate of adsorbents; in vitro action. The exfoliation rate and exfoliation rate of RF were measured by the state elution test. In vitro perfusion simulation experiment, the effect of perfusion on blood components was detected to evaluate the selectivity of adsorbents; the blood compatibility of the prothrombin time (PTs) and thrombin time (TTs) test in vitro was used to verify the blood compatibility of the material. The immunosorbent was used for the rheumatoid factor IgA RF, IgG RF, IgM RF The adsorption rate could be 45.21% + 1.80%, 56.02% + 1.36%, 52.40% + 2.01% (n=5). The abscission rate was 22.10% + 1.65%, 19.23% + 1.06%, 21.31% + 1.35% (n=5) respectively after elution. The adsorption of red blood cells, platelets and total protein were below 10% in the whole blood perfusion test. At the same time, the prothrombin time (PTs) and thrombin time (TTs) in vitro were determined. The results show that PS-PHE can delay the speed of coagulation. Conclusion PS-PHE has high adsorption rate and specificity for RF and shows good blood compatibility. It has a good application prospect in the clinical treatment of rheumatoid arthritis.
【作者单位】：重庆大学生物工程学院生物流变科学与技术教育部重点实验室;第三军医大学第一附属医院中西医结合科风湿病中心;
【基金】：重庆市科委科技计划攻关项目(cstc2011ac5193) 重庆市科委基地建设费(cstc2011pt-gc0030)
【分类号】：R318.08

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