神经干细胞在新型复合支架中的生长和分化

发布时间：2018-06-17 14:30

本文选题：神经干细胞 + 生物相容性材料　；参考：《中国组织工程研究》2016年19期

【摘要】：背景:神经干细胞具有自我增殖和多向分化潜能,是中枢神经组织工程理想的种子细胞。胶原蛋白和丝素蛋白作为生物支架材料已得到广泛应用,但是两者单独使用时均具有不足。那么能否将二者结合起来构建一个新的神经组织工程支架?该新型支架对神经干细胞的生长和分化又会有什么影响呢?目的:观察新型复合支架中神经干细胞的生长和分化情况。方法:将大鼠胚胎神经干细胞接种于新型复合支架中,通过光镜和扫描电镜观察其在支架中生长和分化情况。以常规悬浮培养作为对照组,使用CCK-8检测两组中神经干细胞的活力。对神经干细胞与新型复合支架的三维空间复合体进行石蜡切片,并进行苏木精-伊红染色和免疫荧光染色,观察神经干细胞在材料表面及内部的生长、分化情况。结果与结论:1新型复合支架中的神经干细胞可以很好地生长、分化,细胞与细胞之间形成突触;2CCK-8检测显示,神经干细胞在支架中生长良好,新型复合支架中神经干细胞活力要优于对照组(P0.05);3石蜡切片的苏木精-伊红染色和免疫荧光染色进一步证明神经干细胞在支架中能够很好的生长和分化;4结果说明,制备的新型复合支架具有良好的生物相容性,有利于神经干细胞生长及分化,有良好的应用前景。
[Abstract]:Background: neural stem cells with self-proliferation and multi-differentiation potential are ideal seed cells for central nervous tissue engineering. Collagen and silk fibroin have been widely used as scaffold materials, but they are insufficient when they are used alone. Can we combine the two to construct a new neural tissue engineering scaffold? What effect will the new scaffold have on the growth and differentiation of neural stem cells? Objective: to observe the growth and differentiation of neural stem cells in new composite scaffolds. Methods: rat embryonic neural stem cells were seeded into a new composite scaffold, and the growth and differentiation of neural stem cells in the scaffold were observed by light microscope and scanning electron microscope. Conventional suspension culture was used as control group and CCK-8 was used to detect the activity of neural stem cells in both groups. The three-dimensional space complex of neural stem cells and new composite scaffolds were sectioned with paraffin sections and stained with hematoxylin eosin and immunofluorescence to observe the growth and differentiation of neural stem cells on the surface and inside of materials. Results and conclusion the neural stem cells in the new composite scaffold can grow and differentiate well, and the neural stem cells can grow well in the scaffold. The activity of neural stem cells in the new composite scaffold was better than that in the control group (P 0.05). The results of hematoxylin eosin staining and immunofluorescence staining showed that neural stem cells could grow and differentiate well in the scaffold. The new composite scaffold has good biocompatibility and is beneficial to the growth and differentiation of neural stem cells.
【作者单位】：天津中医药大学;武警后勤学院附属医院脑科医院脑创伤与神经疾病研究所天津市神经创伤修复重点实验室;军事医学科学院卫生装备研究所;
【基金】：天津市科技支撑计划重点项目(14ZCZDGX00500) 武警后勤学院附属医院种子基金项目(FYZ201403)~~
【分类号】：R318.08

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