电化学沉积掺锶透钙磷石涂层的实验研究

发布时间：2018-06-17 22:17

本文选题：锶 + 透钙磷石　；参考：《中国人民解放军医学院》2014年博士论文

【摘要】：研究背景种植体的表面形貌和成分影响种植体的骨结合及种植体长期存活率。纯钛种植体没有诱导成骨的效用，HA涂层不易吸收，有脱壳现象。透钙磷石具有良好的生物相容性，在生理条件下具有较快的降解速率。同时低剂量的锶具有促进骨形成，抑制骨吸收的双重作用。本研究应用电化学沉积法制备了钛基掺锶透钙磷石涂层。希望锶和透钙磷石协同作用，提高种植体骨结合效率，为扩大种植义齿适应症、提高牙种植体成功率提供新的思路。材料和方法 1应用电化学沉积法在纯钛板表面制备钛基掺锶透钙磷石涂层。锶的浓度为0，5%，10%，和20%。应用X射线能谱和X-射线衍射对涂层进行表征分析。通过扫描电子显微镜观察涂层的表面形貌。 2应用电化学沉积法在纯钛种植体表面制备掺锶透钙磷石涂层。将种植体植入SD大鼠胫骨近心端。应用硬组织切片技术和Micro-CT观察掺锶钙磷涂层种植体的骨结合率。 3提取不同比例掺锶透钙磷石涂层的浸提液，在浸提液中培养MC3T3-E1成骨细胞，观察细胞生长情况，应用MTT检测细胞毒性和增殖，ALP检测细胞活性，并应用RT-PCR检测bFGF和VEGF的mRNA表达情况。结果 1通过电沉积法在钛基表面制备出均匀的掺锶透钙磷石涂层，涂层主要由不同形态的晶体组成，主要物相为磷酸氢锶和透钙磷石； 2通过调整不同的锶和钙摩尔比，可得到不同含锶浓度的掺锶透钙磷石涂层。 3锶与透钙磷石协同促进了种植体骨结合； 4掺锶透钙磷石涂层提高了种植体骨结合率；10%掺锶透钙磷石涂层效果显著。 5掺锶透钙磷石涂层促进了成骨细胞ALP的表达； 6掺锶透钙磷石涂层具有较好的安全性和生物相容性； 7掺锶透钙磷石涂层上调了成骨细胞bFGF及VEGF mRNA的表达水平，从而明显增强其对成骨细胞分化的诱导作用； 810%掺锶透钙磷石涂层具有较显著的促进成骨细胞生长的作用。结论：电化学沉积及体内外实验结果表明，电化学沉积掺锶透钙磷石涂层可以促进成骨细胞生长，促进种植体的骨结合。为掺锶透钙磷石涂层种植体的临床应用奠定了理论基础，具有一定的的临床应用价值。
[Abstract]:Background the surface morphology and composition of implants affect bone bonding and long-term survival of implants. Pure titanium implants have no osteogenic effect. Ha coating is not easy to be absorbed and can be dehulled. The permeable phosphorite has good biocompatibility and fast degradation rate under physiological conditions. At the same time, low dose strontium has dual effects of promoting bone formation and inhibiting bone resorption. In this paper, titanium-doped strontium permeable calcium phosphate coating was prepared by electrochemical deposition. It is hoped that the synergistic effect of strontium and pericalciferite could improve the efficiency of implant bone bonding and provide a new idea for expanding the indication of implant denture and improving the success rate of dental implant. Materials and methods 1 Titanium doped strontium permeable calcium phosphate coating was prepared on the surface of pure titanium plate by electrochemical deposition. The concentration of strontium is 0, 5, 10, and 20. The coating was characterized by X ray energy spectrum and X ray diffraction. The surface morphology of the coating was observed by scanning electron microscope. 2 Strontium permeable calcium phosphate coating was prepared on the surface of pure titanium implant by electrochemical deposition. Implants were implanted into the proximal heart of the tibia of SD rats. The bone binding rate of strontium doped calcium phosphate coated implants was observed by hard tissue section technique and Micro-CT. 3 extraction solution with different proportion of strontium doped calcium phosphate coating was extracted, MC3T3-E1 osteoblasts were cultured in the extract, and cell growth was observed. The cytotoxicity and proliferation of ALP were detected by MTT, and the mRNA expressions of bFGF and VEGF were detected by RT-PCR. Results (1) Strontium permeable calcium phosphate coating was prepared on titanium substrate by electrodeposition. The coating mainly consisted of crystals of different morphology, and the main phases were strontium hydrogen phosphate and calcium permeable phosphate. (2) by adjusting the molar ratio of strontium and calcium, strontium permeable phosphor coating with different strontium concentration can be obtained. (4) Strontium permeable calcium phosphate coating improved the bone bonding rate of implant and the effect of 10% strontium permeable calcium phosphate coating was significant. 5 the ALP expression of osteoblasts was promoted by strontium permeable calcium phosphate coating. 6Strontium permeable calcium phosphate coating has good safety and biocompatibility, 7 strontium permeable calcium phosphate coating up-regulates the expression of bFGF and VEGF mRNA in osteoblasts. Thus the induction of osteoblast differentiation was obviously enhanced and 810% strontium permeable calcium phosphate coating could significantly promote the growth of osteoblasts. Conclusion: the results of electrochemical deposition and in vitro and in vivo experiments showed that Sr-doped calcium phosphate coating could promote the growth of osteoblasts and promote the bone bonding of implants. It lays a theoretical foundation for the clinical application of strontium permeable calcium phosphate coated implants and has certain clinical application value.
【学位授予单位】：中国人民解放军医学院
【学位级别】：博士
【学位授予年份】：2014
【分类号】：R783.1

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