纳米羟基磷灰石对成骨细胞系MC3T3-E1的毒性研究

发布时间：2018-06-18 04:31

本文选题：纳米羟基磷灰石 + MC3T3-E1　；参考：《河北大学》2014年硕士论文

【摘要】：随着纳米材料的广泛应用，其潜在的生物安全性引起了科研工作者们广泛关注。纳米羟基磷灰石(HA)作为一种广泛应用于骨组织修复和药物的运载的生物材料，，因此其生物安全性值得人们关注。本文以缺陷发光的HA棒状纳米颗粒作为研究对象，在SEM、XRD、DLS等手段的表征基础之上，以成骨细胞系MC3T3-E1为细胞模型，通过细胞存活率的检测，纳米颗粒的摄取和定位，细胞外乳酸脱氢酶活性的检测，细胞内活性氧表达水平和线粒体膜电位的检测、及细胞凋亡检测等手段在细胞水平上研究了纳米HA对成骨细胞的生物学效应。此外通过DNA凝胶电泳和Caspase-3基因的表达的检测从分子水平上研究了其诱发细胞毒性的机制。结果表明：纳米HA以大胞饮方式进入细胞，主要集中在溶酶体中，少量在线粒体；进入细胞内的纳米颗粒可引起细胞内活性氧水平升高和线粒体膜电位降低，从而抑制细胞增殖。进一步研究表明，纳米HA可引起DNA损伤将细胞周期阻滞在S期，并通过上调Caspase-3基因引起细胞凋亡。
[Abstract]:With the wide application of nanomaterials, their potential biosafety has attracted wide attention of researchers. Nano-hydroxyapatite (HA) is widely used as a biomaterial for bone tissue repair and drug delivery. In this paper, the defect luminescent HA rod nanoparticles were used as the object of study. On the basis of the characterization of SEMX XRDLS, the osteoblast cell line MC3T3-E1 was used as the cell model. The survival rate of the cells and the uptake and localization of the nanoparticles were determined. The biological effects of nano-HA on osteoblasts were studied at the cellular level by detecting the activity of extracellular lactate dehydrogenase, the expression of reactive oxygen species and mitochondrial membrane potential, and the apoptosis of osteoblasts. In addition, the mechanism of cytotoxicity induced by DNA gel electrophoresis and Caspase-3 gene expression was studied at the molecular level. The results showed that the HA nanoparticles entered the cells in the form of large cellular drink, mainly concentrated in lysosomes and a few in mitochondria, and the nanoparticles entering the cells could increase the level of reactive oxygen species and decrease the membrane potential of mitochondria. Thus inhibiting cell proliferation. Further studies showed that nano-HA could induce DNA damage and arrest cell cycle in S phase and induce apoptosis by up-regulating Caspase-3 gene.
【学位授予单位】：河北大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R318.08

【参考文献】