动态多孔海绵结构多层膜负载溶菌酶用于抗菌涂层的研究

发布时间：2018-06-18 07:10

本文选题：动态转变 + 多孔海绵结构　；参考：《高分子学报》2017年05期

【摘要】：以聚丙烯酸(PAA)和聚乙烯亚胺(PEI)为构筑单元,运用层层自组装技术制备了聚电解质多层膜.该多层膜具有独特的动态特点——经酸处理后膜内部形成海绵状通孔结构,该海绵结构在饱和水蒸气的处理下,多孔结构能够闭合,重新回到致密的膜结构.借助该种动态多层膜平台,能够简单有效地通过毛细作用力将溶菌酶负载并固定于多层膜中,为制备基于抗菌蛋白的抗菌涂层提供了新的方法.扫描电镜表征了多层膜动态变化过程,激光共聚焦显微镜表征了溶菌酶在膜内的分布情况,并测定了溶菌酶载入量及其释放动力学.进一步的抗菌测试表明该种抗菌涂层在溶菌酶和PEI的共同作用下可以有效地抑制金黄色葡萄球菌.将多层膜同时负载溶菌酶和乳铁蛋白,提升了涂层对大肠杆菌的杀菌效果.
[Abstract]:Polyelectrolyte multilayer films were prepared by layer-by-layer self-assembly technique using polyacrylic acid (PAA) and polyethyleneimine (PEI) as building units. The multilayer membrane has unique dynamic characteristics: the porous structure is formed in the membrane after acid treatment, and the porous structure can be closed and returned to the dense membrane structure under saturated water vapor treatment. With the help of this dynamic multilayer film platform, lysozyme can be simply and effectively loaded and immobilized in the multilayer film by capillary force, which provides a new method for the preparation of antibacterial coating based on antibacterial protein. The dynamic process of the multilayer membrane was characterized by scanning electron microscope and the distribution of lysozyme in the membrane was characterized by laser confocal microscopy. The loading amount of lysozyme and its release kinetics were measured. Further antimicrobial tests showed that the coating could effectively inhibit Staphylococcus aureus under the combined action of lysozyme and PEI. The multilayer film was loaded with lysozyme and lactoferrin simultaneously to improve the bactericidal effect of the coating on Escherichia coli.
【作者单位】：浙江大学高分子科学与工程学系教育部高分子合成与功能构造教育部重点实验室;
【基金】：浙江省自然科学基金(基金号LR15E030002) 国家重点研发计划专项(项目号2016YFC1102203) 国家自然科学基金(基金号51333005,21374095,51573162)资助项目
【分类号】：TB306;R318.08

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