含透明质酸钠多糖复合防粘连膜的研究

发布时间：2018-06-18 13:00

本文选题：透明质酸钠 + 魔芋葡甘聚糖　；参考：《昆明理工大学》2012年硕士论文

【摘要】：硬膜外粘连的形成被认为是脊柱手术后疼痛的核心因素,并且被认为是压迫神经的最主要原因,而防粘连材料的应用是预防粘连一种有效的方式。一定浓度的透明质酸钠(Sodium hyaluronate,简称SH)可抑制成纤维细胞增殖和运动,在防粘连部位,具有抑制出血、消炎的功效,因此是一种理想的防粘连材料,但其凝胶在体内降解率快,仅在粘连早期有一定的防粘连功效。因此,本课题采用多糖与SH复合制备成共混膜,以期待能够减缓材料的降解,从而长期预防粘连。 1、本论文首先通过透明质酸钠、魔芋葡甘聚糖(Konjac glucomannan,简称KGM)为原料,乳酸作为添加剂,与微波作用共同降低膜液粘度,采用氨水／无水乙醇的混合溶液作为交联剂,制备不可水溶的SH/KGM共混膜；通过正交试验方案L16(45)对SH/KGM共混膜的制备工艺进行了研究。检测结果显示：该共混膜的膜液粘度在290～6267mPa·s,膜厚在41.8-54.5μm之间,拉伸强度与断裂伸长率分别在11.8-17.4MPa、7.1-20.8%之间,吸水率分布151-508%之间,而10周共混膜的降解率在27.1-43.3%之间；并通过对以上结果的综合评定,确定最优方案为魔芋粉2.75g、SH0.09g、乳酸0.9ml、微波加热时间4min、交联时间为4d。该最优组复合膜通过体外细胞培养及兔子体内皮下包埋实验发现SH/KGM共混膜与细胞的粘附性能较弱,但该膜在兔子体内并未导致脓肿、组织变性、坏死和异常增生,因此说明该膜具有一定的生物相容性；通过体外降解实验及体内包埋实验发现该膜的降解率不高,在PBS溶液中的体外降解率35.8%,与纯SH凝胶相比,其降解率大幅度减缓；该复合膜具有较高的吸水率,吸湿后帖附性能好,且吸水后有一定的韧性,可保证在使用过程中不脆断。 2、本论文接着研究了透明质酸钠／壳聚糖(Chitosan,简称CS)／魔芋葡甘聚糖共混膜,以透明质酸钠、壳聚糖为主要原料,KGM作为改性剂,乙酸为添加剂,经充分溶解、减压脱泡、流延成膜、烘干及交联后制备不可溶性共混膜,并判断KGM含量对共混膜性质的影响。 SH/CS/KGM复合膜呈淡黄色,一面粗糙,一面较为光滑；从透光率和扫描电镜图发现,该共混膜具有较好的共混相容性；经研究发现该共混膜具有良好的生物相容性,可支持细胞在膜上生长,且细胞具有良好的形态；但该复合膜的降解率较低,10周的体外降解率仅在5.78%-6.47%。 KGM的加入提高了共混膜的韧性(断裂伸长率在7.1-20.8%),解决了透明质酸钠/壳聚糖共混膜韧性不足的缺点；且提高了共混膜的吸水率,较高的吸水率可吸附损伤部位的组织积液,起到止血的作用,减少瘢痕的形成,从而减轻炎症；但KGM的加入使得共混膜的生物相容性略有降低。
[Abstract]:The formation of epidural adhesion is considered to be the core factor of pain after spinal surgery and the main cause of nerve compression. The application of anti-adhesion materials is an effective way to prevent adhesion. Sodium hyaluronate at a certain concentration can inhibit the proliferation and movement of fibroblasts. In the anti-adhesion site, it has the effect of inhibiting bleeding and anti-inflammation, so it is an ideal anti-adhesion material, but its gel degrades rapidly in vivo. Only in the early stage of adhesion has a certain anti-adhesion effect. Therefore, a blend membrane was prepared with polysaccharide and SH in order to slow down the degradation of the material and prevent adhesion for a long time. 1. In this paper, first of all, through sodium hyaluronate, Konjac glucomannan (KGM), Konjac glucomannan (KGM) was used as the raw material. Lactic acid was used as additive and microwave to reduce the viscosity of membrane liquid. The insoluble SH- / KGM blend membrane was prepared by using mixed solution of ammonia and anhydrous ethanol as crosslinking agent. The preparation process of SH- / KGM blend membrane was studied by orthogonal test scheme L _ (16) ~ (45). The results showed that the viscosity of the blend film was 290V 6267mPa / s, the film thickness was 41.8-54.5 渭 m, the tensile strength and elongation at break were between 11.8-17.4 MPA 7.1-20.8%, the water absorption distribution was 151-508%, and the degradation rate of the blend film was 27.1-43.3% at 10 weeks. Through the comprehensive evaluation of the above results, the optimal scheme was determined as follows: konjac powder 2.75 g / h 0.09 g, lactic acid 0.9 ml, microwave heating time 4 min, crosslinking time 4 d. The results of cell culture in vitro and subcutaneous embedding in rabbits showed that the adhesion of SH- KGM blend membrane to cells was weak, but the membrane did not cause abscess, tissue degeneration, necrosis and abnormal proliferation in rabbits. It was found that the degradation rate of the film was not high in vitro and in vivo, and the degradation rate in PBS solution was 35.8%. Compared with pure SH gel, the degradation rate of the film was much slower than that of pure SH gel. The composite film has high water absorption, good moisture absorption property, and good toughness after water absorption. 2. In this paper, sodium hyaluronate / chitosan Chitosan (CSN / Konjac glucomannan) blend membrane was studied. Sodium hyaluronate and chitosan were used as the main raw materials, and KGM was used as modifier. Acetic acid was used as additive to prepare insoluble blend membrane after fully dissolving, defoaming under pressure, forming film by casting, drying and crosslinking, and the effect of KGM content on properties of blend membrane was evaluated. SHR / CS / KGM composite membrane was yellowish and rough. It was found that the blend film had good compatibility and biocompatibility, which could support the growth of cells on the membrane, and the cells had good morphology, and the blend film had a good compatibility from the light transmittance and scanning electron microscope (SEM), and the study showed that the blend film had a good biocompatibility and the morphology of the cells was good. However, the degradation rate of the composite membrane in vitro was only 5.78-6.47 after 10 weeks of low degradation. The addition of KGM improved the toughness of the blend film (elongation at break was 7.1-20.8%), which solved the disadvantage of insufficient toughness of sodium hyaluronate / chitosan blend film. Moreover, the water absorption rate of the blend membrane was increased, and the higher water absorption rate could adsorb the tissue effusion in the damaged area, which could stop bleeding and reduce the formation of scar, thus reducing inflammation, but the biocompatibility of the blend membrane was slightly decreased with the addition of KGM.
【学位授予单位】：昆明理工大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：TB383.2;R318.08

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