Cultispher微载体构建组织工程软骨修复关节软骨缺损

发布时间：2018-06-18 15:34

本文选题：生物相容性材料 + 水凝胶　；参考：《中国组织工程研究》2017年30期

【摘要】：背景:纤维蛋白胶和微载体均可作为软骨组织工程的良好载体,但因力学性能差和可塑性差等缺点限制其广泛应用。目的:以负载软骨细胞的Cultispher微载体为基础,复合纤维蛋白胶,构建Cultispher微载体/纤维蛋白胶复合支架,观察其用于修复兔膝关节软骨缺损的效果。方法:将兔软骨细胞与Cultispher微载体置于搅拌式生物反应器中三维悬浮共培养,待细胞帖附至微载体表面并大量扩增后,将负载有软骨细胞的Cultispher微载体与纤维蛋白胶复合,构建Cultispher微载体/纤维蛋白胶复合支架,并用于修复兔膝关节股骨滑车软骨缺损。实验按不同的软骨缺损处植入物分为3组:MCF组以负载软骨细胞的Cultispher微载体/纤维蛋白胶复合支架修复软骨缺损;MF组以单纯Cultispher微载体/纤维蛋白胶复合支架修复软骨缺损;空白对照组旷置软骨缺损,不作任何处理。术后3,6个月取材。检测并记录大体观、大体观评分、病理学染色、病理学评分、Micro-CT扫描等指标,评估软骨修复效果。结果与结论:①大体观显示MCF组的软骨修复效果明显优于MF组和空白对照组;②番红"O"、天狼猩红病理染色结果显示MCF组的修复组织主要以透明软骨为主,而MF组和空白对照组的修复组织主要以纤维组织为主;③Micro-CT扫描结果显示,MCF组较MF组和空白对照组获得更好的软骨下骨重建;④MCF组的大体观评分与病理学评分均明显高于MF组和空白对照组;⑤结果证实,负载软骨细胞的Cultispher微载体/纤维蛋白胶复合支架能成功修复兔股骨滑车软骨缺损。
[Abstract]:Background: fibrin glue and microcarriers can be used as good carriers for cartilage tissue engineering, but their wide application is limited by poor mechanical properties and poor plasticity. Aim: to construct Cultispher / fibrin gel composite scaffold based on cultispher microcarrier loaded with chondrocytes and to observe the effect of cultispher / fibrin gel composite scaffold on repairing articular cartilage defects in rabbits. Methods: rabbit chondrocytes and cultispher microcarriers were co-cultured in a stirred bioreactor. The cultispher microcarriers loaded with chondrocytes were mixed with fibrin glue after the cells were attached to the surface of the microcarriers and expanded in large quantities. Cultispher microcarrier / fibrin glue composite scaffold was constructed and used to repair femoral trochlear cartilage defect of rabbit knee joint. The experiment was divided into 3 groups according to the different cartilage defects. The chondrocytes were treated with Cultispher microcarrier / fibrin glue composite scaffold loaded with chondrocytes. In MF group, only Cultispher microcarrier / fibrin glue composite scaffold was used to repair cartilage defect. Blank control group without any treatment. The materials were obtained 3 and 6 months after operation. Gross view, gross grade, pathological staining, pathological score and Micro-CT scan were detected and recorded to evaluate the effect of cartilage repair. Results and conclusion the effect of cartilage repair in MCF group was obviously better than that in MF group and blank control group. The pathological staining of Sirius red showed that the repair tissue of MCF group was mainly hyaline cartilage. The reconstruction of subchondral bone was better in MF group and blank control group than in MF group and blank control group. The results of 3Micro-CT scanning showed that MCF group had better subchondral bone reconstruction than MF group and blank control group. 4the gross and pathological scores of MCF group were significantly higher than those of MF group and blank control group. The results showed that Cultispher microcarrier / fibrin glue composite scaffold loaded with chondrocytes could successfully repair rabbit femoral trochlear cartilage defect.
【作者单位】：长治医学院附属和平医院骨科;解放军总医院骨科;
【分类号】：R318.08;R68

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