组织工程角膜透明度检测方法研究与评价

发布时间：2018-06-23 19:44

本文选题：组织工程角膜 + 透明度　；参考：《中国生物医学工程学报》2014年06期

【摘要】：确定组织工程角膜透明度的测量方法,开发一套能实时测量组织工程角膜透明度的仪器。根据光学的基本原理,以虚拟软件为开发平台,采用计算机,数据采集卡,照度传感器等器件,确定并开发一种检测组织工程角膜透明度的方法与装置,通过对不同光源和被测样品进行检测,评价组织工程角膜透明度测量仪的精度。在确定组织工程角膜透明度测量方法的基础上,完成了基于虚拟仪器技术构建组织工程角膜透明度测量仪器开发,针对可见光、红色激光、有机玻璃、普法玻璃、隐形眼镜和猪角膜的测量计算,确定组织工程角膜透明度测量仪的系统误差修正值为0.009 4,精度误差为0.6%~1.0%。本研究的组织工程角膜透明度测量方法与仪器可以对组织工程角膜进行定量的检测,比目前使用的方法更科学和方便,有望成为组织工程角膜度透明度测量的新标准;采用红色激光光源测量的角膜透明度比普通可见光测得的值有细微不同;但是对于确定的光源,该仪器不影响被测组织工程角膜透明度的优劣评比;更换不同波长光源,可以获得该种波长情况下角膜透明度的精确值。
[Abstract]:To determine the measurement method of corneal transparency in tissue engineering, and to develop a set of instrument to measure the transparency of tissue engineering cornea in real time. According to the basic principle of optics, using virtual software as development platform, using computer, data acquisition card, illuminance sensor and other devices, a method and device for detecting corneal transparency in tissue engineering are determined and developed. The accuracy of the tissue engineering corneal transparency meter was evaluated by measuring the different light sources and the tested samples. On the basis of determining the transparency measurement method of tissue engineering cornea, the development of a tissue engineering corneal transparency measurement instrument based on virtual instrument technology has been completed, aiming at visible light, red laser, plexiglass and universal glass. According to the measurement and calculation of contact lens and porcine cornea, the systematic error correction value of the tissue engineering corneal transparency measuring instrument is 0.009, and the precision error is 0.6 ~ 1.0. The transparency measurement method and instrument of tissue engineering cornea in this study can be used for quantitative measurement of tissue engineering cornea, which is more scientific and convenient than the present method, and it is expected to be a new standard for measuring the transparency of tissue engineering cornea. The corneal transparency measured by the red laser light source is slightly different than that obtained by ordinary visible light. However, for the determined light source, the instrument does not affect the evaluation of the corneal transparency of the tissue engineered cornea, and changes the light source at different wavelengths. The accurate value of corneal transparency at this wavelength can be obtained.
【作者单位】：杭州电子科技大学生命信息与仪器工程学院;复旦大学附属眼耳鼻喉科医院;
【基金】：上海自然科学基金项目(09ZR1432700)
【分类号】：R318

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