RGD肽自组装多层膜修饰钛表面的研究

发布时间：2018-06-24 00:00

本文选题：钛 + RGD肽　；参考：《华中科技大学学报(医学版)》2014年04期

【摘要】：目的利用层层自组装技术,在钛表面构建RGD肽(Arg-Gly-Asp Peptides)多层膜,评价其血液相容性,探讨该技术在医用金属表面改性领域内的应用前景。方法采用氢氧化钠(NaOH)预先处理钛试件,获得多孔、负电荷的钛表面,吸附一层正电荷的聚乙烯亚胺,然后多次交替吸附RGD肽,形成RGD肽的多层膜结构。通过对该涂层进行物理表征测定及体外血液相容性检测,评价其血液相容性。结果 RGD肽自组装多层膜表面水接触角为78.21±1.98°,体外溶血率为0.76%,该涂层还可有效延长部分活化凝血酶原时间(APTT)至(15.1±0.2)s,并且能够有效抑制血小板的粘附与激活。结论利用层层自组装技术形成的RGD肽涂层血液相容性高,有望成为一种新型的生物化钛表面,显示了其在医用金属表面改性领域的广阔应用前景。
[Abstract]:Objective to evaluate the blood compatibility of RGD peptide multilayers on titanium surface by layer self-assembly technique, and to explore its application prospect in the field of medical metal surface modification. Methods the porous and negatively charged titanium surface was prepared with sodium hydroxide (NaOH). A layer of positively charged polyethyleneimine was adsorbed on the surface and then the RGD peptide was adsorbed alternately many times to form the multilayer membrane structure of RGD peptide. The blood compatibility of the coating was evaluated by physical characterization and in vitro blood compatibility test. Results the water contact angle was 78.21 卤1.98 掳and the in vitro hemolysis rate was 0.76. the RGD peptide self-assembled multilayer membrane could also prolong the partial activated prothrombin time (APTT) to (15.1 卤0.2) s, and inhibit platelet adhesion and activation. Conclusion the RGD peptide coating with high blood compatibility formed by layer self-assembly technique is expected to become a new kind of physicochemical titanium surface, which shows its broad application prospect in the field of medical metal surface modification.
【作者单位】：华中科技大学同济医学院附属协和医院心血管外科;
【基金】：国家高新技术研究发展计划(863计划)资助项目(No.2007AA027Z439)
【分类号】：R318.08

【相似文献】