新型组织工程化心脏瓣膜材料的体外生物学性能评价

发布时间：2018-06-25 10:14

本文选题：组织工程 + 心脏瓣膜　；参考：《临床心血管病杂志》2014年01期

【摘要】：目的:评价枝化状聚乙二醇(PEG)交联去细胞猪主动脉瓣的生物相容性。方法:依据GB/T16886.6-1997标准,利用直接接触法和浸提液接触法(间接接触)检测所制备的组织工程化心脏瓣膜材料的致凝血性能、致溶血性能,采用AlamarBlue?细胞活力检测法和Annexin V凋亡检测法评价材料的细胞毒性,并应用形态学方法检测瓣膜表面细胞粘附增殖情况,以新鲜天然瓣膜和去细胞瓣膜作为对照。结果:新型材料不会明显刺激血小板引起其粘附、激活(与天然瓣膜对比,P0.5)。材料接触对凝血功能(INR,APTT)无显著影响。材料直接或间接溶血率均符合生物材料国家标准。AlamarBlue?法检测显示,与材料直接或间接接触对细胞增殖无显著影响,间接接触不增加细胞凋亡概率(P0.1)。细胞接种于材料表面后增殖迅速,形态正常,扫描电镜示细胞连接致密,呈单层排列覆盖材料。结论:枝化状PEG交联去细胞猪主动脉瓣的离体生物相容性达到GB/T16886.6-1997标准,是一种有潜力的组织工程化心脏瓣膜替代材料。
[Abstract]:Objective: to evaluate the biocompatibility of dendritic polyethylene glycol (PEG) crosslinked acellular porcine aortic valve. Methods: according to GB / T 16886.6-1997 standard, direct contact method and extraction solution contact method (indirect contact) were used to detect the coagulation and hemolytic properties of the tissue engineered heart valve materials. Cytotoxicity was evaluated by cell viability assay and Annexin V apoptosis assay. Cell adhesion and proliferation on the surface of the valve were detected by morphological method. Fresh natural valves and acellular valves were used as controls. Results: the new material did not significantly stimulate platelet adhesion and activation (compared with natural valve P 0.5). Material contact had no significant effect on blood coagulation function (INRAPTT). The direct or indirect hemolysis rate of the materials is in accordance with the national standard of biomaterials. The results showed that direct or indirect contact with materials had no significant effect on cell proliferation, and indirect contact did not increase the probability of cell apoptosis (P0.1). After inoculation on the surface of the material, the cells proliferated rapidly and the morphology was normal. SEM showed that the cells were connected and dense, and the cells were arranged in a monolayer arrangement. Conclusion: the biocompatibility of dendritic PEG crosslinked acellular porcine aortic valve in vitro is up to GB / T 16886.6-1997 standard. It is a potential substitute for tissue engineering heart valve.
【作者单位】：华中科技大学附属协和医院心外科;
【基金】：国家863高技术研究发展计划资助项目(No:2009AA03Z420) 国家自然科学基金资助项目(No:30872540;81170214)
【分类号】：R318.08

【参考文献】