盐酸四环素对大鼠骨髓间充质干细胞增殖的作用

发布时间：2018-06-25 23:07

本文选题：干细胞 + 间质干细胞　；参考：《中国组织工程研究》2017年33期

【摘要】：背景:研究表明,低浓度四环素可促进体外培养的人胚成骨细胞活性及增殖,增加且加强细胞合成Ⅰ型胶原、骨钙素等促进骨生成的物质。目的:观察盐酸四环素体外对大鼠骨髓间充质干细胞增殖的作用。方法:取第3代SD大鼠骨髓间充质干细胞,分2组干预,对照组加入含胎牛血清的完全培养基培养,实验组加入含盐酸四环素及胎牛血清的完全培养基培养。培养1-8 d,采用MTT法、细胞计数法检测两组细胞增殖活性。结果与结论:(1)细胞计数法:随着培养时间的延长,两组细胞数量逐渐增加,实验组培养4-8 d的细胞数量高于对照组(P0.05);(2)MTT法:两组细胞数量均随培养时间的延长而增加,第1天为细胞潜伏期,3-6 d为对数生长期,第7天,对照组细胞进入平台期,实验组细胞继续增殖,实验组培养5-8 d的细胞增殖高于对照组(P0.05);(3)结果表明:盐酸四环素可促进骨髓间充质干细胞的增殖。
[Abstract]:Background: low concentrations of tetracycline can promote the activity and proliferation of human embryonic osteoblasts in vitro and increase and enhance the synthesis of collagen type I and osteocalcin. Aim: to observe the effect of tetracycline hydrochloride on proliferation of rat bone marrow mesenchymal stem cells in vitro. Methods: bone marrow mesenchymal stem cells (BMSCs) of the third generation SD rats were divided into two groups: the control group was cultured on the complete medium containing fetal bovine serum and the experimental group was cultured in the complete culture medium containing tetracycline hydrochloride and fetal bovine serum. After 1-8 days of culture, MTT assay and cell count method were used to detect the proliferative activity of the two groups. Results and conclusion: (1) Cell count: with the increase of culture time, the number of cells in the experimental group was higher than that in the control group for 4-8 days (P0.05); (2): the number of cells in both groups increased with the extension of culture time. On the 1st day, the cell incubation period was 3-6 days, which was the logarithmic growth period. On the 7th day, the control group cells entered the plateau phase, and the cells in the experimental group continued to proliferate. The results showed that tetracycline hydrochloride could promote the proliferation of bone marrow mesenchymal stem cells after 5-8 days culture in the experimental group compared with the control group (P0.05); (3).
【作者单位】：遵义医药高等专科学校药学系;四川大学华西医院皮肤科;贵州省骨科医院药剂科;西南交通大学材料先进技术教育部重点实验室材料科学与工程学院;
【基金】：国家自然科学基金(31400809) 贵州省科技厅科学技术基金项目([2012]2366号) 中央高校基本科研业务费专项资金(2682015CX006)~~
【分类号】：R318;R68

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