含石墨烯的聚乳酸复合纳米纤维的制备及细胞相容性

发布时间：2018-06-26 05:57

本文选题：电纺丝 + 石墨烯　；参考：《功能高分子学报》2014年02期

【摘要】：利用电纺丝技术制备了聚乳酸(PLLA)-石墨烯(Gr)复合纳米纤维。通过SEM、TEM及拉曼光谱观察和分析了该复合纳米纤维的形貌及Gr在PLLA中的分散状态,通过DSC、TG、力学拉伸及接触角测试等表征方法研究了PLLA-Gr复合纳米纤维的热性能、力学性能、亲水性等物理性能,最后,通过MTT法检测一种神经胶质细胞——雪旺细胞(SCs)在该复合纳米纤维支架上的生长和增殖行为,评价其细胞相容性。结果表明:低含量的Gr能较好地分散在PLLA纳米纤维中,且随着m(Gr):m(PLLA)从0增加到2.0%,所得到的纤维直径呈现先减小后增加的趋势。当m(Gr):m(PLLA)增加到1.0%时,PLLA-Gr纳米纤维的直径达到最小,为(0.50±0.19)μm,对应的拉伸强度较未添加Gr时增加也最大达50%。PLLA-Gr纳米纤维膜的结晶度与Gr的含量和分散性有关。细胞培养1、4、7 d后的MTT检测结果表明PLLA-Gr复合纳米纤维能促进SCs的黏附和生长。PLLA-Gr复合纳米纤维具有较好的细胞相容性。
[Abstract]:Polylactic acid (PLLA)-graphene (Gr) nanofibers were prepared by electrospinning. The morphology of the composite nanofiber and the dispersion of Gr in PLLA were observed and analyzed by SEM TEM and Raman spectroscopy. The thermal and mechanical properties of PLLA-Gr nanofiber were studied by DSC-TG, mechanical tensile and contact angle measurements. Finally, the growth and proliferation of Schwann cells (SCs) on the composite nanofiber scaffold were measured by MTT assay. The results show that the low content of Gr can be dispersed in PLLA nanofibers, and with the increase of m (Gr): M (PLLA) from 0 to 2.0, the diameter of the obtained fibers decreases first and then increases. When m (Gr): M (PLLA) increased to 1.0, the diameter of PLLA-Gr nanofibers reached the minimum (0.50 卤0.19) 渭 m, the corresponding tensile strength increased to 50% and the maximum value was 50%. The crystallinity of PLLA-Gr nanofibers was related to the content and dispersion of Gr. MTT assay showed that PLLA-Gr nanofibers could promote the adhesion and growth of SCs. PLLA-Gr composite nanofibers had good cytocompatibility.
【作者单位】：东华大学化学化工与生物工程学院;
【基金】：国家自然科学基金(51073032) 上海市科学技术委员会‘浦江人才计划'(10PJ1400200) 中央高校基本科研业务费专项资金资助项目(11D10540,13D110523) 教育部留学回国人员科研启动基金项目(11W10523)
【分类号】：R318.08

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