纳米APS外膜肝素化内膜小口径组织工程血管的实验研究

发布时间：2018-06-27 11:21

本文选题：组织工程血管 + 脱细胞　；参考：《上海交通大学学报(医学版)》2017年03期

【摘要】：目的·制备具有良好抗张强度及组织相容性的复合型组织工程血管(HTEV)。方法·制备大鼠脱细胞主动脉血管支架,采用静电纺丝(ES)技术在脱细胞血管支架外覆以纳米氨基醇聚癸二酸甘油酯(APS),使用肝素修饰血管支架内膜,制成HTEV。行HTEV体内植入和功能评价,建立同种异体的大鼠腹主动脉移植模型。移植后6周行血管超声和CT血管造影检查。结果·大鼠主动脉经脱细胞处理后,细胞成分几乎被完全去除,脱细胞组的总DNA含量[(115.4±10.9)ng/mg]相较未脱细胞组[(398.6±14.6)ng/mg]显著下降(P=0.000),但脱细胞对于胶原纤维以及弹力纤维造成损伤。ES-APS包覆后增强了HTEV支架的机械性能,支架壁厚(187±11)μm、缝合强度为(0.51±0.06)N、爆破压力为(2 103±232)mm Hg,均较脱细胞血管显著提高(均P0.01)。HTEV内膜经肝素修饰后具有较强的抗血小板黏附作用。大鼠异体血管移植后6周检查发现,HTEV通畅性良好,包覆ES-APS能防止扩张和动脉瘤形成。结论·通过在大鼠脱细胞主动脉血管支架外覆以ES-APS,内膜以肝素修饰,成功制备了HTEV,大大提高了抗张强度及组织相容性。
[Abstract]:Objective to prepare composite tissue engineering vessels (HTEV) with good tensile strength and histocompatibility. Methods the stents of rat acellular aorta were prepared. The stents were coated with nano-APS and heparin were used to modify the intima of the stents. HTEV was prepared by electrospinning (es) technique. An allograft model of abdominal aorta was established by in vivo implantation and functional evaluation of HTEV. Ultrasound and CT angiography were performed 6 weeks after transplantation. Results the cellular components of rat aorta were almost completely removed after acellular treatment. The total ng/mg content in acellular group [(115.4 卤10.9) ng/mg] was significantly lower than that in non-acellular group [(398.6 卤14.6) ng/mg] (P0. 000). The wall thickness of the stent was (187 卤11) 渭 m, the suture strength was (0.51 卤0.06) N, and the blasting pressure was (2 103 卤232) mm Hg, which was significantly higher than that of the acellular vessels (P 0.01). Six weeks after allograft transplantation, HTEV was found to be smooth in rats, and coated with ES-APS could prevent dilatation and aneurysm formation. Conclusion HTEV was successfully prepared by adding ES-APS to the aorta stents and heparin modified endometrium, which greatly improved the tensile strength and histocompatibility.
【作者单位】：苏州大学附属第三医院心胸外科;
【分类号】：R318.11

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