婴儿皮肤组织成纤维细胞系的建立及生物学特性

发布时间：2018-06-27 15:56

本文选题：婴儿皮肤 + 成纤维细胞系　；参考：《南京师大学报(自然科学版)》2014年03期

【摘要】：采集婴儿皮肤组织,用组织块法制备原代细胞,通过差速消化和差速贴壁法纯化细胞,扩大培养至第3代液氮冻存,复苏细胞后进行活力、形态、生长曲线、微生物污染、核型、乳酸脱氢同工酶谱及荧光蛋白质粒转染表达等特性研究.结果显示,婴儿皮肤组织细胞呈成纤维型,生长状态良好;生长曲线呈"S"型,最大增值浓度为1.64×105/mL,倍增时间为63.12 h;细菌、真菌、支原体检测均为阴性;染色体2n=46,二倍体为86%,占主体;乳酸脱氢酶同工酶电泳图谱显示有5条谱带;外源质粒在该细胞中能进行复制和表达.表明已成功建立了婴儿皮肤组织成纤维细胞系,这为复合人工皮肤的组织工程学研究奠定了前期工作基础,也为皮肤相关疾病的研究提供了实验条件,因而具有重要的理论和实践意义.
[Abstract]:The skin tissues of infants were collected and primary cells were prepared by tissue block method. The cells were purified by differential digestion and differential adherence method. The cells were expanded to the third generation of liquid nitrogen cryopreservation. After resuscitation, the cells were resuscitated for vitality, morphology, growth curve and microbial contamination. Karyotype, lactate dehydrogenation isozyme and fluorescent protein transfection and expression were studied. The results showed that the skin tissue cells of infant had fibroblast type, and the growth curve was "S" type, the maximum increment concentration was 1.64 脳 10 ~ 5 / mL, the doubling time was 63.12 h, and the detection of bacteria, fungi and mycoplasma were all negative. Chromosome 2nnc46, diploid 86.Lactic dehydrogenase isozyme electrophoresis showed that there were five bands, and exogenous plasmids could be duplicated and expressed in the cell. The results indicated that the infantile skin tissue fibroblast cell line had been successfully established, which laid a foundation for the study of tissue engineering of composite artificial skin and provided experimental conditions for the study of skin related diseases. Therefore, it has important theoretical and practical significance.
【作者单位】：甘肃省动物细胞工程技术研究中心;西北民族大学生命科学与工程学院;
【基金】：西北民族大学中青年科研基金
【分类号】：R318.08

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