聚二甲基硅氧烷基材表面修饰及力学强度变化对关节软骨细胞的影响

发布时间：2018-06-28 22:12

  本文选题：组织工程 + 软骨　； 参考：《中国组织工程研究》2017年14期

【摘要】：背景:聚二甲基硅氧烷因具有良好的生物相容性,优异的微纳米尺度可加工性,被广泛应用于细胞生物学的基础研究。但聚二甲基硅氧烷材料疏水性强,并不适于细胞贴附,因此表面修饰非常重要。目的:考察不同表面修饰方法并结合聚二甲基硅氧烷材料力学强度变化对牛关节软骨细胞生物学行为的影响。方法:采用3种不同方法修饰聚二甲基硅氧烷薄膜材料,包括胎牛血清孵育、胶原沉积和等离子体处理,同时变化聚二甲基硅氧烷材料的硬度,然后接种牛关节软骨细胞,并通过细胞骨架染色以及CCK-8表征分析其黏附、增殖情况,通过天狼星红/番红O染色以及糖胺聚糖定量表征分析基质分泌情况。结果与结论:(1)细胞黏附和增殖:细胞骨架染色以及生长曲线可以看出血清孵育、胶原沉积和等离子体处理均能显著改善牛关节软骨细胞在聚二甲基硅氧烷表面的黏附和增殖,其中等离子体处理的效果最好;(2)糖胺聚糖的总量:只有等离子体处理的表面显著提高了细胞分泌的糖胺聚糖的总量,但是细胞分泌糖胺聚糖的能力在所有修饰的表面上有所降低;(3)材料硬度对细胞黏附、生长和糖胺聚糖分泌都有影响,而且与材料表面修饰有关。(4)结果证实:实验利用血清孵育、胶原沉积以及等离子体处理聚二甲基硅氧烷材料,均能有效促进牛关节软骨细胞的贴附和生长,而且其中等离子体处理效果最优,与此同时聚二甲基硅氧烷基材的硬度也对细胞行为有所影响,但程度上要次于化学修饰,而且越能促进细胞生长的材料越抑制了关节软骨细胞的基质分泌能力。
[Abstract]:Background: Poly (dimethylsiloxane) has been widely used in the basic research of cell biology because of its good biocompatibility and excellent micro- and nanoscale processability. However, polydimethylsiloxane is not suitable for cell adhesion because of its strong hydrophobicity, so surface modification is very important. Aim: to investigate the effects of different surface modification methods and mechanical strength of polydimethylsiloxane on the biological behavior of bovine articular chondrocytes. Methods: polydimethylsiloxane membrane materials were modified by three different methods, including fetal bovine serum incubation, collagen deposition and plasma treatment, and the hardness of polydimethylsiloxane materials was changed at the same time, and then bovine articular chondrocytes were inoculated. The adhesion and proliferation were analyzed by cytoskeleton staining and CCK-8 staining. The matrix secretion was analyzed by Sirius red / vanillin O staining and glycosaminoglycan quantitative analysis. Results and conclusion: (1) Cell adhesion and proliferation: cytoskeleton staining and growth curve showed that serum incubation, collagen deposition and plasma treatment could significantly improve the adhesion and proliferation of bovine articular chondrocytes on the surface of polydimethylsiloxane. The effect of plasma treatment was the best. (2) the total amount of glycosaminoglycan: only the surface treated by plasma significantly increased the total amount of glycosaminoglycan secreted by cells. However, the ability of cells to secrete glycosaminoglycan was decreased on all the modified surfaces. (3) the hardness of the material affected cell adhesion, growth and glycosaminoglycan secretion, and was related to the surface modification of the material. (4) the results showed that the experiment was incubated with serum. Collagen deposition and plasma treatment of polydimethylsiloxane can effectively promote the adhesion and growth of bovine articular chondrocytes, and the plasma treatment is the best. At the same time, the hardness of polydimethylsiloxane also affected the cell behavior, but to a lesser extent than the chemical modification, and the more the material can promote cell growth, the more inhibit the matrix secretion ability of articular chondrocytes.
【作者单位】： 华东理工大学生物反应器工程国家重点实验室;
【基金】：上海市自然科学基金项目(16ZR1408700)~~
【分类号】：R318.08;R68
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本文编号：2079551