基于多光谱成像技术的生物标志物快速检测方法研究

发布时间：2018-06-29 05:12

本文选题：生物标志物 + 多光谱成像技术　；参考：《合肥工业大学》2017年硕士论文

【摘要】：生物标志物是一类能够反映人体疾病、自然环境等相关情况的信号指标,在疾病预判与诊断、药物疗效评估、环境暴露评估等领域具有广泛应用。头发由于便于收集、运输方便、性质稳定、保存成本低、易于被接受等优点,已被广泛认可为评估疾病、环境、药物和毒物的理想生物标志物。头发暴露于皮肤的最外层,相对于皮肤其更易受到紫外辐照,因此头发可以作为紫外辐照评估的靶器官。紫外会对头发造成损伤,从而头发的光损伤程度可以用于评估人体接收的紫外辐照量。基于多光谱成像技术,本文通过分段研究的方式对头发的光损伤进行定性分析,并通过电子扫描电镜进行表征。研究结果表明,生长时间越长的头发具有更严重的光损伤程度,其平均光谱反射值在近红外区域也显著升高。在进一步实验中,我们将同一段头发在紫外灯下辐照特定时间并测定辐照前后的胱氨酸含量。研究结果表明,紫外辐照可以精确再现生长不同时间的头发段的光谱反射值;且辐照后头发的胱氨酸含量减小,表明胱氨酸含量的损失是导致头发纤维表面形貌变化,从而导致头发光谱反射值的变化的主要原因。在另一方面,头发中的重金属含量有助于评估人体重金属暴露及体内重金属含量水平。本文基于多光谱成像技术,研究了头发纤维表面的重金属累积。研究结果表明,头发表面重金属累积越多,在505 nm~700 nm处的光谱反射值越低;且头发的损伤程度越严重,吸附重金属离子的能力越强。本工作表明多光谱成像技术可以应用于头发光损伤和重金属累积的快速、无损评估,为建立模型评估紫外辐照及重金属累积程度提供了可行性,并且为后期通过多光谱成像技术检测头发以评估人体的重金属含量、环境暴露、药物和毒物暴露等提供了理论基础,对于促进人体健康研究具有重要意义。
[Abstract]:Biomarker is a kind of signal index which can reflect the related situation of human body disease, natural environment and so on. It is widely used in the field of disease prediction and diagnosis, drug efficacy evaluation, environmental exposure assessment and so on. Hair has been widely recognized as an ideal biomarker for evaluating diseases, environment, drugs and poisons because of its advantages such as easy collection, convenient transportation, stable nature, low preservation cost and easy acceptance. Hair exposed to the outermost layer of the skin is more susceptible to UV irradiation than the skin, so hair can be used as a target organ for UV radiation evaluation. UV can damage the hair, so the degree of light damage can be used to evaluate the UV radiation received by the human body. Based on the multispectral imaging technique, the light damage of hair was analyzed qualitatively and characterized by electron scanning electron microscope (SEM). The results show that the longer the growth time, the more serious the light damage, and the higher the average spectral reflectance is in the near infrared region. In further experiments, the same hair was irradiated under ultraviolet lamp for a specific time and the cystine content was measured before and after irradiation. The results show that UV irradiation can accurately reproduce the spectral reflectance of hair growth at different time, and the cystine content of hair decreases after irradiation, which indicates that the loss of cystine content leads to the change of the surface morphology of hair fiber. As a result, the main reason for the change of the spectral reflectance of hair. On the other hand, the content of heavy metals in hair helps to assess heavy metal exposure and heavy metal levels in the body. The accumulation of heavy metals on the surface of hair fibers was studied based on multispectral imaging technique. The results show that the higher the accumulation of heavy metals on the hair surface, the lower the spectral reflectance at 505 nm~700 nm, and the more serious the damage is, the stronger the ability to adsorb heavy metal ions. This work shows that multispectral imaging can be applied to the rapid and nondestructive assessment of hair light damage and heavy metal accumulation, which provides a feasible model for the assessment of UV irradiation and heavy metal accumulation. It also provides a theoretical basis for the assessment of heavy metal content, environmental exposure, drug and poison exposure through multispectral imaging technology, which is of great significance to the study of human health.
【学位授予单位】：合肥工业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R312

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