聚甲基丙烯酸甲酯骨水泥对肺癌细胞毒性作用的体外实验

发布时间：2018-07-03 08:17

本文选题：聚甲基丙烯酸类 + 椎体成形术　；参考：《中国组织工程研究》2017年02期

【摘要】：背景:经皮椎体成形是治疗脊柱转移瘤的一种微创手术方法,但术中注射的聚甲基丙烯酸甲酯(polymethylmethacrylate,PMMA)骨水泥对肿瘤的治疗机制目前尚不明确。目的:分析PMMA骨水泥及其单体对肿瘤细胞的细胞毒作用。方法:将固化期、拉丝期PMMA骨水泥的浸提液及不同质量浓度单体的稀释液与人肺腺癌细胞spc-A1接触培养1,3 d后,采用细胞形态直接观察法和CCK-8比色法测定细胞吸光度(A)值,计算细胞相对增殖率(RGR),评价PMMA骨水泥及其单体对肿瘤细胞的毒性。结果与结论:11 d 1 g/L组、10 g/L组的A值明显小于阴性对照组(P0.01);3 d 1 g/L组、10 g/L组及拉丝期浸提液A值明显小于阴性对照组(P0.05);2结果显示培养1 d 1 g/L组、10 g/L组的A值明显小于阴性对照组(P0.01),细胞相对增殖率为26%-29%,毒性评价为4级;3 d 1 g/L组、10 g/L组的A值明显小于阴性对照组,细胞相对增殖率12%-16%,毒性评价为4至5级;3 d 100 mg/L组、拉丝期PMMA浸提液组的A值明显小于阴性对照组,毒性评价为2级。3结果表明,拉丝期PMMA骨水泥浸提液及PMMA单体质量浓度为100 mg/L-10 g/L时对肿瘤细胞有明显的毒性,说明经皮椎体成形过程中拉丝期PMMA骨水泥注入椎体后其释放的单体可能对肿瘤细胞存在细胞毒作用。
[Abstract]:Background: percutaneous vertebroplasty is a minimally invasive method for the treatment of spinal metastases, but the mechanism of intraoperative injection of polymethylmethacrylate (PMMA) bone cement for the treatment of tumors remains unclear. Objective: to analyze the cytotoxic effect of PMMA bone cement and its monomer on tumor cells. Methods: the extractions of PMMA bone cement and the diluents of different concentrations of monomers were cultured with spc-A1 for 1 to 3 days during the curing and drawing stages. The (A) values of the cells were measured by direct observation of cell morphology and CCK-8 colorimetry. The relative cell proliferation rate (RGR) was calculated to evaluate the toxicity of PMMA bone cement and its monomer to tumor cells. Results and conclusion the A value of 10 g / L group was significantly lower than that of the negative control group (P0.01) 3 d 1 g / L group, the A value of the 10 g / L group and the extraction fluid in the drawing stage was significantly lower than that of the negative control group (P0.05). The results showed that the A value of 1 day 1 g / L group was significantly lower than that of the 10 g / L group (P0.01) and the A value of 10 g / L group was significantly lower than that of the negative control group (P0.05). The relative proliferation rate of cells was 26-29. The A value of 10 g / L group was significantly lower than that of negative control group, and the toxicity evaluation was 3 days after 1 g / L group, and the A value in 10 g / L group was significantly lower than that in negative control group. The relative proliferation rate of cells was 12- 16. The toxicity evaluation was 4 to 5 grade 3 d 100 mg / L group. The A value of PMMA extract group was significantly lower than that of negative control group. The toxicity evaluation showed that the toxicity was grade 2. 3. PMMA bone cement extract and PMMA monomer concentration of 100 mg / L ~ (-10) g / L had obvious toxicity to tumor cells in the drawing stage, and the concentration of PMMA monomer was 100 mg / L ~ (-10) g / L. It is suggested that the monomer released by PMMA bone cement injected into the vertebra during percutaneous vertebroplasty may have cytotoxic effect on tumor cells.
【作者单位】：北京大学第一医院骨科;
【分类号】：R734.2;R318.08

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