超临界流体技术制备聚乳酸组织工程三维多孔支架实验研究

发布时间：2018-07-03 19:18

  本文选题：超临界流体 + 组织工程　； 参考：《功能材料》2017年05期

【摘要】：理想三维支架的构建是组织工程化人工器官成功的前提条件。以聚乳酸为模型材料,二氯甲烷为溶剂,利用超临界流体诱导相分离过程制备具有外部三维立体结构和内部三维互通的组织工程支架,考察了过程制备的可行性,分析了操作参数如操作压力、温度和溶液浓度对孔的形态、结构和孔壁的影响。结果表明,利用超临界流体诱导相分离过程可成功制备孔径分布均匀、互通性好、孔隙率高的组织工程用三维多孔支架,且制备周期短,工艺简单。随操作压力、温度和溶液浓度的增加,支架孔径均逐渐减小。但过程中温度太低,压力或初始溶液浓度太高均会导致聚合物富相内聚乳酸的快速结晶析出从而形成由纳米级聚乳酸颗粒粘结在一起的支架孔壁。本实验范围内较优的操作参数为:高压釜压力10 MPa,温度45℃,溶液浓度20%(质量分数)。
[Abstract]:The construction of ideal three-dimensional scaffold is a prerequisite for the success of tissue engineering artificial organs. Using polylactic acid as model material and dichloromethane as solvent, tissue engineering scaffolds with external three-dimensional structure and internal three-dimensional interworking were prepared by supercritical fluid-induced phase separation process. The effects of operating parameters such as operating pressure, temperature and solution concentration on the pore morphology, structure and pore wall were analyzed. The results show that three-dimensional porous scaffolds for tissue engineering with uniform pore size distribution, good interoperability and high porosity can be successfully prepared by using supercritical fluid-induced phase separation process, and the preparation period is short and the process is simple. With the increase of operating pressure, temperature and solution concentration, the pore diameter of the scaffold decreases gradually. However, when the temperature is too low, the pressure or the initial solution concentration is too high, the rapid crystallization of poly (lactic acid) in the rich phase of the polymer will lead to the formation of the scaffold pore wall bonded by nano-sized polylactic acid particles. The optimum operating parameters are as follows: the pressure of autoclave is 10 MPA, the temperature is 45 鈩，

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