荧光材料增强契伦科夫光学信号的探索研究

发布时间：2018-07-08 21:17

本文选题：契伦科夫光学成像 + 四环素　；参考：《第三军医大学学报》2017年22期

【摘要】：目的探究四环素、荧光素钠、异硫氰酸荧光素(fluorescein isothiocyanate,FITC)这3种临床可用的荧光材料对契伦科夫光学信号增强的效果及最佳应用方案。方法将不同浓度的四环素溶液(100、50、10、5、1、0.5、0.1、0.05、0.01 mmol/L)、FITC溶液(5、4、3、2.5、1.5、1、0.5、0.25 mmol/L)和荧光素钠溶液(100、50、10、5、2、1、0.2、0.1 mmol/L)分别与100μCi的~(18)F-FDG混合后进行契伦科夫光学成像(Cerenkov luminescence imaging,CLI),观察契伦科夫光学信号的增强效果,并勾画感兴趣区域(region of interest,ROI),对光学信号强度进行定量分析,确定最佳信号增强效果的浓度,并对信号最强的板孔以20 nm为间隔进行500~800 nm范围的光谱分离成像。同时进行在体实验验证荧光素钠及FITC对契伦科夫光的增强效果。结果不同浓度的四环素溶液对光学信号无明显影响。FITC及荧光素钠均能显著提高契伦科夫光学信号强度。当FITC及荧光素钠浓度分别为1或2 mmol/L时产生的光学信号强度最强,为单独的~(18)F-FDG的3.2倍及5倍。光谱分析表明2 mmol/L的荧光素钠能将~(18)F-FDG产生的契伦科夫光(cerenkov luminescence,CL)光红移至540 nm附近。荷瘤鼠在体实验表明注射FITC或荧光素钠后能使肿瘤部位的CL信号强度提高2倍或3.9倍。结论 FITC、荧光素钠均能通过契伦科夫光的激发效应来增强契伦科夫光学信号强度,提高组织穿透力。
[Abstract]:Objective to investigate the effect of tetracycline, sodium fluorescein and fluorescein isothiocyanate on the enhancement of Cherenkov optical signal. Methods the different concentrations of tetracycline solution (100 ~ (50) C ~ (10) ~ (0.5) ~ (18) F-FDG) were mixed with 100 渭 ci ~ (18) F-FDG to observe the enhancement effect of Cerenkov luminescence imagingCLI, and the enhancement effect of Cerenkov luminescence imagingCLI was observed by observing the enhancement effect of Cerenkov optical signal, respectively, after mixing 100 渭 ci ~ (18) F-FDG with 100 ~ (50) 渭 ci ~ (18) F-FDG, respectively, and sodium fluorescein (100 ~ (50) 105210.22 ~ (0.1) mmol 路L ~ (-1) ~ (18) F ~ (18) F-FDG), respectively, in order to observe the enhancement of Cerenkov luminescence imagingCLI, and to observe the enhancement effect of Cerenkov luminescence imagingin CLI (100 ~ (18) F-FDG). The region of interest (region of) was drawn, the intensity of optical signal was quantitatively analyzed, the concentration of the best signal enhancement effect was determined, and the spectral separation imaging of the strongest plate hole was carried out in the range of 500-800 nm at an interval of 20 nm. At the same time, the enhancement effect of fluorescein sodium and FITC on Cherenkov light was tested in vivo. Results different concentrations of tetracycline solution had no significant effect on optical signal. FITC and sodium fluorescein could significantly improve the intensity of Cherenkov optical signal. When the concentration of FITC and sodium fluorescein was 1 or 2 mmol / L, the intensity of optical signal was the strongest, which was 3.2 and 5 times of that of ~ (18) F-FDG alone. Spectral analysis shows that 2 mmol / L sodium fluorescein can shift the cerenkov luminescence CL produced by ~ (18) F-FDG to about 540nm. In vivo experiments in tumor-bearing mice showed that FITC or sodium fluorescein could increase the signal intensity of CL in tumor site by 2 times or 3.9 times after injection of FITC or sodium fluorescein. Conclusion both FITC and sodium fluorescein can enhance the intensity of optical signal and enhance the penetration of tissue through the excitation effect of Cherenkov light.
【作者单位】：武警总医院消化内科;
【基金】：国家自然科学基金面上项目(81471700)~~
【分类号】：R817

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