煅烧异种骨的生物相容性研究

发布时间：2018-07-10 03:57

本文选题：煅烧异种骨 + 细胞毒性　；参考：《中国修复重建外科杂志》2017年10期

【摘要】：目的探讨煅烧异种骨材料的生物相容性,为其应用于临床提供实验依据。方法取健康成年牛松质骨,采用脱蛋白、高温煅烧方法制备煅烧异种骨。将煅烧异种骨材料浸提液与L929细胞体外共同培养,于第2、4、7天评价其细胞毒性。L929细胞接种至煅烧异种骨,培养4 d后扫描电镜观察细胞在材料表面贴附及增殖情况,评价其细胞相容性。于10只新西兰大白兔双侧后肢胫骨钻孔,左侧孔内植入煅烧异种骨(实验组),右侧植入羟基磷灰石生物陶瓷(对照组);术后4、26周取材行大体观察及组织学观察,评价煅烧异种骨生物相容性。结果细胞毒性实验显示,煅烧异种骨浸提液细胞毒性为0~1级。细胞相容性实验显示,L929细胞在煅烧异种骨表面贴附良好,并向孔隙内部生长。体内植入实验显示4周时两组均有轻度或中度炎性反应,可见新骨形成;26周时两组均无炎性反应且有不同程度新骨形成。结论煅烧异种骨具有良好生物相容性,有望用于临床骨缺损修复。
[Abstract]:Objective to investigate the biocompatibility of calcined xenogenic bone materials and to provide experimental evidence for its clinical application. Methods healthy adult bovine cancellous bone was prepared by deproteinization and high temperature calcinations. The extractant of calcined xenogeneic bone was cultured with L929 cells in vitro, and the cytotoxicity of L929 cells was evaluated on the 2nd day. L929 cells were inoculated to calcined xenogeneic bone. After 4 days of culture, the adhesion and proliferation of L929 cells on the surface of the materials were observed by scanning electron microscope (SEM). To evaluate its cytocompatibility. Ten New Zealand white rabbits were drilled in the tibia of bilateral hind limbs, the calcined xenograft was implanted in the left hole (experimental group) and hydroxyapatite bioceramics was implanted in the right side (control group), and the gross and histological observation were performed at 426 weeks after operation. To evaluate the biocompatibility of calcined xenogeneic bone. Results Cytotoxicity test showed that the cytotoxicity of calcined xenogenic bone extract was 0 ~ 1 grade. The cytocompatibility test showed that the L929 cells adhered well to the surface of calcined xenograft bone and grew into the pore. In vivo implantation experiment showed that there were mild or moderate inflammatory reaction in both groups at week 4, and no inflammatory reaction and different degree of new bone formation were found in both groups at 26 weeks after new bone formation. Conclusion calcined xenogeneic bone has good biocompatibility and is expected to be used in clinical bone defect repair.
【作者单位】：中国辐射防护研究院山西省组织库山西奥瑞生物材料有限公司;
【分类号】：R318.08;R68

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