肠外瘘封堵可降解抗菌水凝胶的制备及体外性能

发布时间：2018-07-20 10:02

【摘要】：背景:目前对于肠外瘘的外科手术治疗手段有待改进,寻求一种人工合成水凝胶代替纤维蛋白凝胶作为封堵材料势在必行。目的:制备出可注射可降解抗菌水凝胶材料,并检测其体外生物学性能。方法:以水溶性壳聚糖(S-Cts)和氧化海藻酸钠(O-Alg)为原料,采用席夫碱反应制备原位成型凝胶,在此基础上加入氧化还原反应得到的纳米银(nano-Ag)颗粒,形成S-Cts/O-Alg/nano-Ag复合水凝胶。观测凝胶时间和微观形貌,通过模拟生理环境溶液,检测复合水凝胶的溶胀性能、降解性能以及抗菌性能。结果与结论:①水溶性壳聚糖与氧化海藻酸钠的组分含量越接近,凝胶时间越短,并可以控制在外科手术所需范围内;②2种组分含量的变化会影响形成凝胶的微观结构形貌,水溶性壳聚糖组分含量越大,凝胶网络结构越致密;③复合凝胶具备优秀的溶胀性能,在含有胰蛋白酶的模拟肠液中相比在磷酸盐缓冲溶液中降解速率更快;④凝胶组分中加入纳米银微粒,有效提升了凝胶的抗菌性能;⑤这种材料弥补了单纯天然高分子材料的生物相容性、生物可降解性以及在抑菌能力上的不足,具备一定研究价值和应用前景。
[Abstract]:Background: at present, the surgical treatment of intestinal fistula needs to be improved. It is imperative to seek a synthetic hydrogel instead of fibrin gel as a plugging material. Objective: to prepare injectable biodegradable hydrogel material and to detect its biological properties in vitro. Methods: using water-soluble chitosan (S-Cts) and sodium alginate (O-Alg) as raw materials, the in-situ gel was prepared by Schiff base reaction. On this basis, the nano-silver (nano-Ag) particles obtained by redox reaction were added to form S-Cts-O-Alg / nano-Ag composite hydrogel. The swelling, degradation and antibacterial properties of the composite hydrogels were measured by simulating the physiological environment solution by observing the gel time and micromorphology. The results showed that the closer the content of water-soluble chitosan to sodium alginate was, the shorter the gel time was, and the change of 22 components could affect the microstructure and morphology of the gel. The larger the content of water-soluble chitosan, the denser the gel network is, the better the swelling property of the composite gel is, and the degradation rate is faster in the simulated enteric solution containing trypsin than in the phosphate buffer solution. (4) the addition of nano-silver particles into the gel component can effectively improve the antibacterial properties of the gel. This material makes up for the lack of biocompatibility, biodegradability and bacteriostatic ability of pure natural polymer materials. It has certain research value and application prospect.
【作者单位】：南京理工大学材料科学与工程学院;
【基金】：国家自然科学基金(51575278) 江苏省科技计划项目(BY2016004-08,BA2015054)~~
【分类号】：R318.08

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