低能量激光照射离体高血脂全血和红细胞对其吸收光谱的影响

发布时间：2018-07-24 14:32

【摘要】：高血脂是诱发心脑血管疾病和动脉血管粥样硬化等多种疾病的重要因素,因此调节体内血脂水平是治疗高血脂疾病的关键环节。对于血脂的治疗,临床上有抽脂手术、针灸治疗、药物治疗、激光治疗等治疗方法。其中激光治疗效果显著,目前还没有发现副作用。自从20世纪90年代之后,国内开始出现低能量激光照射疗法(Low-levelLaserTherapy,LLLT),此疗法可对生物体的组织代谢系统、免疫系统以及血液循环系统进行调节,其原理是激光所产生的生物学效应,从而可以使患者从病理状态恢复到正常状态。本实验利用低能量激光照射对生物组织作用产生生物效应,激光照射离体血液后,观察全血以及红细胞对光谱吸收的变化,来分析血脂的改变,从而为低能量激光照射改善高血脂人群的血脂水平提供新的思路和方法。目的:本实验研究在650nm和405nm激光照射高血脂血样和正常血样的离体全血和红细胞,观察照射前后全血和红细胞对光谱吸收的变化。方法:选取医院中用全自动生化分析仪测出的30例高血脂血样和30例血脂正常血样各4ml,将正常血样设为正常组,高血脂血样设为高血脂组。高血脂组与正常组分别用激光波长650nm和405nm照射,调整激光参数,使激光器输出功率密度为20mW/cm2,激光能量密度为12J/cm2,照射15min,吸取0.5ml进行红细胞分离,分离的红细胞与剩下的0.5ml全血样本稀释之后用酶标仪进行光谱扫描。用多功能酶标仪扫描全血以及全血分离的红细胞并绘制图谱,统计全血及红细胞吸收光谱的特性,比较正常组与高血脂组,激光照射前与照射后全血和红细胞吸收光谱的不同之处,观察吸收峰值的变化。结果:使用650nm激光照射高血脂组,对于全血统计中,照射后光谱吸收峰,比照射前高,在吸收峰416nm、544nm、578nm处,照射前后差异表现出统计学意义。对于红细胞统计中,照射后红细胞光谱吸收峰在416nm、544nm、578nm比照射前降低,且照射前后差异表现出统计学意义,其中在吸收峰416nm差异更为突出。使用650nm激光照射正常组,对于全血与红细胞统计中,照射后红细胞光谱吸收峰在416nm、544nm、578nm照射前后差异无统计学意义。使用405nm激光照射仅对高血脂红细胞吸收光谱产生差异,对正常全血、红细胞以及高血脂全血均未对其吸收光谱产生显著性差异。结论:通过650nm与405nm低能量激光照射可以降低高血脂血液中红细胞对光谱的吸收,间接反映出低能量激光在治疗高血脂方面的疗效。
[Abstract]:Hyperlipidemia is an important factor to induce cardiovascular and cerebrovascular diseases and atherosclerosis, so regulating the level of blood lipid is the key link in the treatment of hyperlipidemia. For the treatment of blood lipids, clinical liposuction surgery, acupuncture treatment, drug therapy, laser treatment and other treatment methods. Among them laser treatment effect is remarkable, no side effects have been found at present. Since the 1990s, Low-level LaserTherapyne LLLT (LLLT) has been introduced in China, which can regulate the tissue metabolism system, immune system and blood circulatory system of organism, which is based on the biological effect of laser. Thus, the patient can recover from pathological state to normal state. In this experiment, we used low energy laser irradiation to produce biological effect on biological tissue. After laser irradiation on isolated blood, we observed the changes of spectral absorption of whole blood and erythrocytes to analyze the changes of blood lipids. Therefore, it provides a new idea and method for low energy laser irradiation to improve the blood lipid level of hyperlipidemia population. Aim: to study the effects of 650nm and 405nm laser irradiation on whole blood and red blood cells of hyperlipidemia and normal blood samples, and observe the changes of spectral absorption of whole blood and red blood cells before and after irradiation. Methods: 30 cases of hyperlipidemia blood samples and 30 cases of normal blood samples of blood lipid were measured by automatic biochemical analyzer in hospital. The normal blood samples were divided into normal group and hyperlipidemia blood sample as hyperlipidemia group. In hyperlipidemia group and normal group, laser wavelength 650nm and 405nm were used to adjust laser parameters to make the laser output power density 20 MW / cm 2, laser energy density 12 J / cm 2, irradiation for 15 min, and 0.5ml was absorbed to separate red blood cells. The isolated red blood cells were diluted with the remaining 0.5ml whole blood samples and then scanned by an enzymatic spectrometer. The red blood cells separated from whole blood and whole blood were scanned by multifunctional enzyme standard instrument, and the characteristics of absorption spectrum of whole blood and red blood cell were analyzed and compared between normal group and hyperlipidemia group. The difference between the absorption spectra of whole blood and red blood cells before and after laser irradiation was observed. Results: in the whole blood statistics, the absorption peak after irradiation was higher than that before irradiation, and the difference before and after irradiation was statistically significant at 416nm ~ 544nm ~ 578nm. In erythrocyte statistics, the absorption peak of red blood cell at 544 nm after irradiation was lower than that before irradiation, and the difference before and after irradiation was statistically significant, especially in the absorption peak 416nm. In the whole blood and erythrocyte statistics, there was no significant difference between the whole blood and red blood cells before and after irradiation with 650nm laser irradiation at 416nm ~ 544nm ~ 578nm. The absorption spectra of hyperlipidemia red blood cells were only different by 405nm laser irradiation, but there was no significant difference on the absorption spectra of normal whole blood, red blood cells and hyperlipidemia whole blood. Conclusion: low energy laser irradiation with 650nm and 405nm can reduce the absorption of red blood cells in hyperlipidemia and indirectly reflect the therapeutic effect of low energy laser on hyperlipidemia.
【学位授予单位】：北京协和医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R589.2;R454.2

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