蜂窝状聚氨酯支架的制备及性能评价

发布时间：2018-07-28 17:30

【摘要】：以2,2'-二硫代二乙醇为引发剂,经开环聚合法合成聚ε-己内酯二元醇,该大分子二元醇再与1,6-己二异氰酸酯加成聚合得到聚酯型聚氨酯,利用红外光谱仪、差示扫描量热仪和X射线衍射仪对合成聚氨酯结构和性能进行表征;然后,利用生物模板法制备出蜂窝状聚氨酯支架,分别用扫描电子显微镜、基于阿基米德原理的测量方法和万能材料试验机表征支架形貌、孔隙率和压缩性质;最后,通过小鼠成骨细胞MC3T3-E1与支架共培养法评价支架细胞相容性。结果表明,合成聚氨酯分子结构得到验证,呈半结晶性特点;所得聚氨酯支架呈现典型的蜂窝状孔结构,孔径范围为100~140μm,孔隙率为77.2%±4.1%,压缩模量为299.02 kPa;肌动蛋白染色和死活细胞染色结果说明该蜂窝状聚氨酯支架能支持MC3T3-E1细胞黏附、生长和增殖,细胞形态良好。该蜂窝状聚氨酯支架在骨组织工程中具有良好的应用前景。
[Abstract]:Poly (蔚 -caprolactone) diol was synthesized by ring-opening polymerization with 2o _ 2o _ 2-dithioethanol as initiator. The macromolecular diol was added to 1 ~ (6) -caprodiisocyanate to form polyester polyurethane. The structure and properties of polyurethanes were characterized by differential scanning calorimetry (DSC) and X-ray diffractometer (XRD). The measurement method based on Archimedes principle and the universal material testing machine were used to characterize the morphology, porosity and compression properties of the scaffolds. Finally, the biocompatibility of the scaffolds was evaluated by co-culture of mouse osteoblasts MC3T3-E1 and scaffolds. The results showed that the molecular structure of synthetic polyurethane was verified, and the structure of polyurethane scaffold was characterized by typical honeycomb pore structure. The pore size was 100 渭 m, the porosity was 77.2% 卤4.1, and the compression modulus was 299.02 KPA. The results of actin staining and living cell staining showed that the honeycomb polyurethane scaffold could support the adhesion, growth and proliferation of MC3T3-E1 cells and the morphology of the cells was good. The honeycomb polyurethane scaffold has a good prospect in bone tissue engineering.
【作者单位】：西安交通大学金属材料强度国家重点实验室;
【基金】：国家自然科学基金(50773062,50603020)
【分类号】：R318.08

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