基于UDA-2的膜片钳系统程序的设计与实现
发布时间：2018-07-29 08:28
【摘要】：膜片钳技术现在已经成为电生理领域的主要技术手段，与分子生物学、细胞生物学、分子遗传学、生理学、病理学和药理学等学科都有着密切联系。国际上领先的膜片钳系统正在向高通量、工业化、全自动化的方向发展。目前世界上首个拥有48通道的高通量全自动膜片钳系统是由丹麦的Sophion公司推出的QPatch HT系统。它每天可记录250~1200个数据物。目前我们使用的是PC2C型膜片钳放大器，与之配套的有UDA-1型/UDA-2型数据采集系统，和膜片钳系统控制软件PClamp4.0。我的主要工作是在膜片钳系统控制软件PClamp4.0的基础上设计并调试应用程序使之与新研发的UDA-2型数据采集系统匹配。本文主要论述了UDA-2数据采集系统的特点和优势，以及针对UDA-2数据采集系统设计的模块，和详细的实现方法。 本文首先以整体的膜片钳系统结构入手，，介绍膜片钳系统的软件、硬件组成部分。以应用程序内核框架为主，分别介绍内核框架中刺激波形产生模块，刺激波形发送数据采集模块、数据处理模块、端口旋钮监控模块等七个模块的功能，以及各个模块之间的相互关系。针对UDA-2数据采集系统，详细说明数据系统的启动、刺激波形产生模块、刺激波形发送数据采集模块以及端口监控模块的功能具体实现过程。 升级的应用程序在调试过程中出现几个问题：兼容UDA-1型和UDA-2型这两种数据采集系统的方法、显示的数据波形出现毛刺以及调试中出现编译错误。本文中对错误原因进行分析，给出相应的解决方案。最后对全文内容进行总结，并对未来进行展望。
[Abstract]:Patch clamp technology has become the main technology in the field of electrophysiology, and has a close relationship with molecular biology, cell biology, molecular genetics, physiology, pathology and pharmacology. The world's leading patch-clamp systems are developing towards high throughput, industrialization, and full automation. The world's first 48-channel high-throughput automatic patch clamp system is the QPatch HT system introduced by Denmark's Sophion Company. It can record between 250 and 1200 data a day. At present, we use the PC2C patch-clamp amplifier, with the UDA-1 / UDA-2 data acquisition system, and the patch clamp system control software PClamp4.0. My main work is to design and debug the application program based on the patch-clamp system control software PClamp4.0 to match the newly developed UDA-2 data acquisition system. This paper mainly discusses the characteristics and advantages of UDA-2 data acquisition system, as well as the design module of UDA-2 data acquisition system and the detailed implementation method. In this paper, the whole structure of patch clamp system is introduced, and the software and hardware of patch clamp system are introduced. Based on the kernel framework of application program, the functions of seven modules, such as stimulation waveform generation module, stimulating waveform sending data acquisition module, data processing module, port knob monitoring module and so on, are introduced respectively. And the relationship between each module. For the UDA-2 data acquisition system, the function realization process of the data system starting, stimulating waveform generating module, stimulating waveform sending data acquisition module and port monitoring module is described in detail. There are several problems in the debugging process of the upgraded application: the methods of compatible UDA-1 and UDA-2 data acquisition system, the burr of the displayed data waveform and the compilation errors in debugging. In this paper, the causes of errors are analyzed, and the corresponding solutions are given. Finally, the full text of the content of the summary, and prospects for the future.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.6;TP274.2
本文编号：2152089
[Abstract]:Patch clamp technology has become the main technology in the field of electrophysiology, and has a close relationship with molecular biology, cell biology, molecular genetics, physiology, pathology and pharmacology. The world's leading patch-clamp systems are developing towards high throughput, industrialization, and full automation. The world's first 48-channel high-throughput automatic patch clamp system is the QPatch HT system introduced by Denmark's Sophion Company. It can record between 250 and 1200 data a day. At present, we use the PC2C patch-clamp amplifier, with the UDA-1 / UDA-2 data acquisition system, and the patch clamp system control software PClamp4.0. My main work is to design and debug the application program based on the patch-clamp system control software PClamp4.0 to match the newly developed UDA-2 data acquisition system. This paper mainly discusses the characteristics and advantages of UDA-2 data acquisition system, as well as the design module of UDA-2 data acquisition system and the detailed implementation method. In this paper, the whole structure of patch clamp system is introduced, and the software and hardware of patch clamp system are introduced. Based on the kernel framework of application program, the functions of seven modules, such as stimulation waveform generation module, stimulating waveform sending data acquisition module, data processing module, port knob monitoring module and so on, are introduced respectively. And the relationship between each module. For the UDA-2 data acquisition system, the function realization process of the data system starting, stimulating waveform generating module, stimulating waveform sending data acquisition module and port monitoring module is described in detail. There are several problems in the debugging process of the upgraded application: the methods of compatible UDA-1 and UDA-2 data acquisition system, the burr of the displayed data waveform and the compilation errors in debugging. In this paper, the causes of errors are analyzed, and the corresponding solutions are given. Finally, the full text of the content of the summary, and prospects for the future.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.6;TP274.2
【参考文献】
相关期刊论文 前4条
1 艾平,郑建全;膜片钳技术的新进展及其在药物高通量筛选中的应用[J];生理科学进展;2005年02期
2 肖魁;张廷攀;;基于SHARC处理器的同步FIFO传输设计与应用[J];声学与电子工程;2007年04期
3 许庆功;李孜;赵佳;张永胜;;高速异步FIFO的设计与实现[J];微计算机信息;2009年20期
4 李雯,周永超,瞿安连;基于CPLD的膜片钳数据采集系统——UDA-I数据采集器[J];现代科学仪器;2005年04期
相关硕士学位论文 前5条
1 程祥;钙离子浓度荧光显微检测系统中控制模块的设计[D];华中科技大学;2011年
2 邹侨;新型膜片钳系统软件的研制[D];华中科技大学;2007年
3 秦彩杰;基于多线程事件同步技术的钙离子浓度检测软件的设计[D];华中科技大学;2007年
4 李文;可复用面向对象的膜片钳系统软件设计[D];华中科技大学;2007年
5 盛安康;膜片钳系统软件设计[D];华中科技大学;2008年
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