毛囊来源的神经嵴干细胞修复大鼠坐骨神经缺损
发布时间：2018-08-02 18:18
【摘要】：毛囊来源的神经嵴干细胞(Epidermal Neural Crest Stem Cell,EPI-NCSC)由于取材方便,具有多种分化潜能,是一种具有良好应用前景的组织工程种子细胞。目前,在神经损伤修复领域中,EPI-NCSC主要被应用于脊髓损伤的修复。为了探讨EPI-NCSC对周围神经缺损的修复作用,对原代培养的GFP-SD大鼠来源的EPI-NCSC的体外性质进行了考察,并以其为种子细胞,将其等量与细胞外基质(Extracellular matrix,ECM)混合后,预置入聚乳酸-聚羟基乙酸共聚物(Poly lactic acid co glycolic acid copolymer,PLGA)导管中,同时,以等量的达尔伯克(氏)改良伊格尔(氏)培养基(Dulbecco's Modified Eagle's medium,DMEM)代替EPI-NCSC作为对照,以用于修复大鼠坐骨神经10 mm距离的缺失。噻唑蓝(Methyl thiazolyl tetrazolium,MTT)比色分析结果显示,EPI-NCSC在PLGA膜上的初期粘附率为89.7%。在第1、3、5、7天细胞相对增殖率分别为89.3%、87.6%、85.6%和96.6%。细胞周期与DNA倍体分析表明,与PLGA共培养的EPI-NCSC与单独培养的EPI-NCSC相比较,二者的细胞周期变化趋势相同,增殖指数变化趋势也相同。在神经导管移植4周,术部实现了组织学水平的修复。大鼠手术一侧后肢感觉功能有所恢复,坐骨神经指数有所提高。研究结果表明,在PLGA导管中预置EPI-NCSC,有望实现较好的周围神经缺损的修复效果。
[Abstract]:The neural cristal stem cell (Epidermal Neural Crest Stem Cell-EPI-NCSC) derived from hair follicle is a kind of tissue engineering seed cell with good application prospect because of its convenient material collection and multiple differentiation potential. At present, EPI-NCSC is mainly used in the repair of spinal cord injury in the field of nerve injury repair. In order to investigate the effect of EPI-NCSC on repairing peripheral nerve defects, the in vitro properties of EPI-NCSC derived from primary cultured GFP-SD rats were investigated. The same amount of EPI-NCSC was mixed with the extracellular matrix (Extracellular) as seed cells. A polylactic acid-poly-glycolic acid copolymer (Poly lactic acid co glycolic acid copolymerium PLGA) catheter was prepositioned. At the same time, EPI-NCSC was replaced by a modified (Dulbecco's Modified Eagle's medium (DMEM) of the same amount as Darbek's medium. To repair the loss of 10 mm distance of sciatic nerve in rats. The results of (Methyl thiazolyl tetrazolium MTT colorimetric analysis showed that the initial adhesion rate of EPI-NCSC on PLGA membrane was 89.7%. On the 7th day, the relative proliferation rate of the cells was 87.6% and 96.6%, respectively. Cell cycle and DNA ploidy analysis showed that compared with EPI-NCSC co-cultured with PLGA and EPI-NCSC cultured alone, the cell cycle change trend was the same and proliferation index change trend was the same. After 4 weeks of nerve conduit transplantation, the histological level was restored in the surgical department. The sensory function and sciatic nerve index of one hind limb of the rats were recovered and the sciatic nerve index was improved. The results show that EPI-NCSC-preimplantation in PLGA catheter is expected to achieve a better repair effect of peripheral nerve defect.
【作者单位】： 西南大学生命科学学院;淡水鱼类资源与生殖发育教育部重点实验室;第三军医大学大坪医院野战外科研究所创伤、烧伤与复合伤国家重点实验室;重庆医科大学生理学教研室神经科学研究中心;
【基金】：国家重点基础研究发展计划(973计划)(No.2012CB518106) 创伤、烧伤与复合伤国家重点实验室Ⅰ类研究课题(No.SKLZZ201003) 国家自然科学基金(Nos.31271036,81371341)资助~~
【分类号】：R318;Q813
[Abstract]:The neural cristal stem cell (Epidermal Neural Crest Stem Cell-EPI-NCSC) derived from hair follicle is a kind of tissue engineering seed cell with good application prospect because of its convenient material collection and multiple differentiation potential. At present, EPI-NCSC is mainly used in the repair of spinal cord injury in the field of nerve injury repair. In order to investigate the effect of EPI-NCSC on repairing peripheral nerve defects, the in vitro properties of EPI-NCSC derived from primary cultured GFP-SD rats were investigated. The same amount of EPI-NCSC was mixed with the extracellular matrix (Extracellular) as seed cells. A polylactic acid-poly-glycolic acid copolymer (Poly lactic acid co glycolic acid copolymerium PLGA) catheter was prepositioned. At the same time, EPI-NCSC was replaced by a modified (Dulbecco's Modified Eagle's medium (DMEM) of the same amount as Darbek's medium. To repair the loss of 10 mm distance of sciatic nerve in rats. The results of (Methyl thiazolyl tetrazolium MTT colorimetric analysis showed that the initial adhesion rate of EPI-NCSC on PLGA membrane was 89.7%. On the 7th day, the relative proliferation rate of the cells was 87.6% and 96.6%, respectively. Cell cycle and DNA ploidy analysis showed that compared with EPI-NCSC co-cultured with PLGA and EPI-NCSC cultured alone, the cell cycle change trend was the same and proliferation index change trend was the same. After 4 weeks of nerve conduit transplantation, the histological level was restored in the surgical department. The sensory function and sciatic nerve index of one hind limb of the rats were recovered and the sciatic nerve index was improved. The results show that EPI-NCSC-preimplantation in PLGA catheter is expected to achieve a better repair effect of peripheral nerve defect.
【作者单位】： 西南大学生命科学学院;淡水鱼类资源与生殖发育教育部重点实验室;第三军医大学大坪医院野战外科研究所创伤、烧伤与复合伤国家重点实验室;重庆医科大学生理学教研室神经科学研究中心;
【基金】：国家重点基础研究发展计划(973计划)(No.2012CB518106) 创伤、烧伤与复合伤国家重点实验室Ⅰ类研究课题(No.SKLZZ201003) 国家自然科学基金(Nos.31271036,81371341)资助~~
【分类号】：R318;Q813
【共引文献】
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