掺锶硫酸钙及DBM复合材料骨修复能力评价

发布时间：2018-08-13 12:25

【摘要】：[目的]制备一种掺锶硫酸钙与DBM复合的骨修复材料,为临床提供一种成骨活性优良的骨修复材料。[方法]制备掺锶硫酸钙与DBM复合的活性骨修复材料,其中锶元素的量为钙含量的6%,掺锶硫酸钙与DBM按体积比1∶1复合。观察兔股骨髁骨缺损模型修复效果,利用X线片观察缺损区域的新骨形成情况,采用MicroCT评价局部的骨量和骨质变化,对组织切片予以Van Gieson(VG)复合染色法染色进行组织形态学观察,荧光双标观测计算成骨速率。[结果]术后动物无死亡,所有兔子切口无渗血或出现分泌物状况,术后活动正常。从MicroCT的BV/TV结果看出实验组在12周时缺损区域已经恢复;掺锶CaSO_4复合材料组缺损部位已经基本修复,CaSO_4组未完全修复。VG组织学观察显示各组均有新生骨小梁生成,荧光双标观测得到的矿化沉积率显示复合材料组新骨生长突出,矿化沉积率为(2.31±0.13)μm/d。[结论]通过MicroCT、VG组织学染色以及荧光双标观测对兔子股骨髁缺损修复效果进行评价,发现复合材料具有较好的骨缺损修复能力。
[Abstract]:[objective] to prepare a composite bone repair material of calcium strontium sulfate and DBM, and to provide a bone repair material with excellent osteogenic activity. [methods] the composite of strontium calcium sulfate and DBM was prepared, in which the content of strontium was 6% of calcium, and the volume ratio of strontium calcium sulfate and DBM was 1:1. The repair effect of rabbit femoral condylar bone defect model was observed. The new bone formation in the defect area was observed by X-ray film. The local bone mass and bone change were evaluated by MicroCT, and the histomorphology was observed by Van Gieson (VG) compound staining. The rate of osteogenesis was calculated by fluorescence double labeling. [results] there was no death, no blood leakage or secretion in all rabbit incisions, and normal postoperative activity. According to the BV/TV results of MicroCT, the defect area was recovered in the experimental group at 12 weeks, and the defect site in the strontium doped CaSO_4 composite group was basically repaired. The histopathological observation showed that the new bone trabeculae were formed in all groups. The mineralized deposition rate of the composite group was (2.31 卤0.13) 渭 m / d. [conclusion] the results of microCTVG staining and fluorescence double labeling were used to evaluate the repair effect of femoral condylar defect in rabbits. It was found that the composite material had better repairing ability of bone defect.
【作者单位】：中国医科大学附属第一医院骨科;辽宁省北票市中心医院骨科;辽宁医学院附属第一医院骨科;解放军总医院第一附属医院北京市骨科植入医疗器械工程技术研究中心;
【基金】：国家自然科学基金项目(编号:81201380) 军事医学项目(编号:13CXZ028,AWS14C007) 北京市自然科学基金(编号:7153174,7152144) 北京市科技新星项目(编号:Z1511000003150134) 临床部重点项目(编号:2014ZD001)
【分类号】：R318.08

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