结直肠癌患者血液表面增强拉曼光谱研究

发布时间：2018-08-18 12:43

【摘要】：表面增强拉曼光谱技术(SERS)是当今国际生物医学领域的热点研究技术,本论文根据结直肠癌临床诊断需求,将具有高生物检测灵敏度的SERS技术应用于结直肠癌患者血液的分析,并结合医学统计方法,试图发展一种无损、快速、客观、准确的基于SERS技术的结直肠癌血液检测方法。主要研究工作包括： 1.研究以银纳米颗粒为增强基底的结直肠癌病人血液的SERS光谱,通过对比38个结直肠癌患者与45个健康人的血液SERS光谱并结合多元统计方法,对二者的光谱进行区分与判别,诊断灵敏度和特异性分别为81.6%和75.6%。 2.将蛋白膜电泳技术与基于银纳米颗粒的SERS技术相结合,试图从血液蛋白SERS的角度探索结直肠癌检测的可能性。结果显示：利用血清白蛋白SERS光谱来区分结直肠癌患者与健康人的诊断方法的灵敏度和特异性分别为84.2%和80%;而血清球蛋白则为84.2%和75.6%。结果表明：电泳提纯的血清蛋白的诊断效果优于全血清SERS的诊断效果,进一步提高了血液SERS的结直肠癌诊断效果。 3.提出了一种利用接收光偏振SERS技术检测结直肠癌血液的新方法。判别结果显示：当接收光路上的偏振片竖直(0°)、水平(90°)放置时,结直肠癌血清的检测灵敏度分别为89.5%和100%；特异性分别为93.3%和84.4%。结果表明：基于接收光偏振SERS技术的结直肠癌血清诊断效果明显优于常规SERS的诊断效果。 4.根据金纳米颗粒更具生物兼容性的特点,研究以金纳米颗粒为增强基底的结直肠癌病人血液的SERS光谱,通过对比结直肠癌患者与健康人的血液SERS光谱并结合多元统计方法,对二者的光谱进行区分与判别,诊断灵敏度和特异性分别达到97.4%和100%,诊断效果要明显优于银胶SERS。结果表明：利用基于金纳米粒子的SERS技术并结合LDA-PCA的光谱判别诊断法对癌症血清进行分析,是一种新颖的、无损的、极具潜力的结直肠癌检测及筛选手段。
[Abstract]:Surface enhanced Raman spectroscopy (SERS) is a hot research technique in the field of biomedicine. According to the requirement of clinical diagnosis of colorectal cancer, the SERS technique with high sensitivity of biological detection is applied to the analysis of the blood of colorectal cancer patients. Combined with the medical statistical method, this paper attempts to develop a nondestructive, rapid, objective and accurate blood detection method for colorectal cancer based on SERS technology. The main research work includes: 1. The SERS spectra of the blood of colorectal cancer patients with silver nanoparticles as the enhancement substrate were studied. By comparing the SERS spectra of 38 colorectal cancer patients with 45 healthy people and combining with the multivariate statistical method, the spectra of the two groups were distinguished and distinguished. The diagnostic sensitivity and specificity were 81.6% and 75.6%, respectively. In order to explore the possibility of detection of colorectal cancer from the point of view of blood protein SERS, the technique of protein membrane electrophoresis was combined with SERS technology based on silver nanoparticles. The results showed that the sensitivity and specificity of the method were 84.2% and 80%, respectively, and those of serum globulin were 84.2% and 75.6%, respectively. The results showed that the diagnostic effect of serum protein purified by electrophoresis was better than that of whole serum SERS, and further improved the diagnostic effect of blood SERS for colorectal cancer. A new method for detecting the blood of colorectal cancer by receiving light polarization SERS is proposed. The discriminant results showed that when the polarizer was placed vertically (0 掳) and horizontally (90 掳), the detection sensitivity was 89.5% and 100%, and the specificity was 93.3% and 84.4%, respectively. The results showed that the diagnostic effect of SERS based on receiving light polarization was significantly better than that of conventional SERS. 4. According to the more biocompatible characteristics of gold nanoparticles, the SERS spectra of the blood of colorectal cancer patients based on gold nanoparticles were studied. By comparing the SERS spectra of the patients with colorectal cancer and healthy people and combining the multivariate statistical method, The diagnostic sensitivity and specificity were 97.4% and 100% respectively, and the diagnostic effect was obviously better than that of silver gel SERS. The results showed that the SERS technique based on gold nanoparticles and the spectral discriminant diagnostic method based on LDA-PCA were a novel, non-destructive and potential method for detection and screening of colorectal cancer.
【学位授予单位】：福建师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：O433;R735.3;R318.51

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