丝素蛋白-聚乙烯醇复合凝胶对骨组织工程支架性能的影响

发布时间：2018-08-18 15:43

【摘要】：组织工程骨支架材料及制备对支架性能影响至关重要。同单一聚乙烯醇凝胶相比,丝素蛋白-聚乙烯醇复合凝胶不仅可以增加支架所承受应力而且可以提高支架韧性。研究证明,使用水浴加热方法,将丝素蛋白粉末与聚乙烯醇晶粒按照1∶4混合加热,制成丝素蛋白-聚乙烯醇复合凝胶。再与羟基磷灰石充分混合,基于低温冷冻干燥技术,制备成试验试样。压缩试验测量最大压缩强度为31.2 MPa,弹性模量为11.07 MPa,最大总变形低于单一聚乙烯醇/羟基磷灰石试样,且压缩时不易发生断裂及粉碎,综合力学性能较好。电镜实验观察得到羟基磷灰石/丝素蛋白-聚乙烯醇复合材料试样孔径为15~350μm,微孔分布均匀且连通性较好。水浴加热后的丝素蛋白-聚乙烯醇(1∶4)复合凝胶与羟基磷灰石按照1.5∶1混合时,3D打印复合材料支架成形好,无丝材膨胀,孔隙明显。使用此方法制备的羟基磷灰石/丝素蛋白-聚乙烯醇复合材料,力学性能及孔径大小利于负重部位骨缺损修复及骨细胞的生长。
[Abstract]:The material and preparation of tissue engineering bone scaffold is very important to the performance of scaffold. Compared with the single polyvinyl alcohol gel, the composite gel of silk fibroin and polyvinyl alcohol can not only increase the stress of the scaffold, but also improve the toughness of the scaffold. It was proved that silk fibroin / polyvinyl alcohol composite gel was prepared by heating silk fibroin powder and polyvinyl alcohol grain at 1:4 by water bath heating. The test samples were prepared by mixing with hydroxyapatite and freeze-drying at low temperature. The maximum compressive strength and elastic modulus are 31.2 MPA and 11.07 MPA respectively. The maximum total deformation is lower than that of the single PVA / hydroxyapatite sample, and the fracture and comminution are not easy to occur during compression, and the comprehensive mechanical properties are better. The pore size of hydroxyapatite / silk fibroin polyvinyl alcohol composite was 15 ~ 350 渭 m, the micropore distribution was uniform and the connectivity was good. When the silk fibroin-polyvinyl alcohol (1:4) composite gel and hydroxyapatite were mixed with 1.5: 1 after heating in water bath, the 3D printing composite scaffold was well formed, no silk material expanded, and the porosity was obvious. The mechanical properties and pore size of hydroxyapatite / silk fibroin polyvinyl alcohol composites prepared by this method are beneficial to the repair of bone defects and the growth of bone cells.
【作者单位】：新疆大学机械工程学院;西安交通大学机械工程学院;
【基金】：国家自然科学基金资助项目(51665055) 自治区高校科研计划项目(XJEDU2016I016) 机械制造系统国家重点实验室开放基金项目(Sklms20160011) 自治区青年科技创新人才培养工程项目(gn2015yx008)
【分类号】：R318.08;R68

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