流动剪切力促小口径组织工程血管内皮化
发布时间：2018-08-20 19:34
【摘要】：背景:细胞内皮化的流动剪切力大小和作用方式是小口径组织工程血管的构建中的关键问题。小口径组织工程血管构建后的抗血流冲击能力是决定移植成功的关键因素之一,较多人把研究重点集中在如何制作移植血管支架材料,如何提高宿主细胞种植后其血管内壁的抗凝效果,而对细胞内皮层的力学驯化则关注较少,该方法有可能是提高组织工程血管移植成功率的有效手段之一。目的:比较单一水平和成梯度增加的流动剪切力对组织工程血管内皮化的影响,获得适用于组织工程血管内皮化的剪切力大小和加载方式。方法:采用血管铺片硝酸银染色法和内皮细胞肌动蛋白荧光标记法,观察组织工程血管内壁上内皮细胞的数量、形状和黏附性能等变化情况。结果与结论:经2.5和3.0 N/m~2剪切力作用2 h后,血管内壁上的内皮细胞几乎全部脱落;而经0.5-3.0 N/m~2,每2 h增加0.2 N/m~2的成梯度增加的剪切力作用后,血管内皮细胞层保持较完整,细胞形态成明显的轴向排列,且能诱导内皮细胞中应力纤维的出现。说明成梯度增加的流动剪切力这种"驯化"方式,能较好地促进小口径组织工程血管的内皮化。
[Abstract]:Background: the size and mode of flow shear force of endothelial cells are the key problems in the construction of small diameter tissue engineering blood vessels. The ability of small caliber tissue engineering blood vessel to resist blood flow impact is one of the key factors to determine the success of transplantation. How to improve the anticoagulant effect of host cells after implantation, but less attention to the mechanical acclimation of the intracellular cortex, this method may be one of the effective methods to improve the success rate of tissue engineering vascular transplantation. Aim: to compare the effects of single level and gradient increased flow shear stress on vascular endothelization in tissue engineering, and obtain the shear force and loading mode suitable for tissue engineering vascular endothelialization. Methods: the number, shape and adhesion of endothelial cells on the inner wall of tissue engineered vessels were observed by silver nitrate staining and fluorescence labeling of actin in endothelial cells. Results and conclusion: after 2 h and 3.0 N/m~2 shear stress, almost all of the endothelial cells on the inner wall of the vessel shed off, but after the addition of 0.2 N/m~2 / 2 of shear stress every 2 h, the vascular endothelial cell layer remained intact. The morphology of the cells formed an obvious axial arrangement and induced the appearance of stress fibers in endothelial cells. The results show that the "acclimation" of flow shear force in gradient can promote the endothelialization of small caliber tissue engineering vessels.
【作者单位】： 冠昊生物科技股份有限公司再生型医用植入器械国家工程实验室;广东省体育科学研究所;
【基金】：广东省重大科技专项(2010A080407005) 国家重点研究计划生物医用材料研发与组织器官修复替代重点专项(2016YFC1103202)~~
【分类号】：R318.08
[Abstract]:Background: the size and mode of flow shear force of endothelial cells are the key problems in the construction of small diameter tissue engineering blood vessels. The ability of small caliber tissue engineering blood vessel to resist blood flow impact is one of the key factors to determine the success of transplantation. How to improve the anticoagulant effect of host cells after implantation, but less attention to the mechanical acclimation of the intracellular cortex, this method may be one of the effective methods to improve the success rate of tissue engineering vascular transplantation. Aim: to compare the effects of single level and gradient increased flow shear stress on vascular endothelization in tissue engineering, and obtain the shear force and loading mode suitable for tissue engineering vascular endothelialization. Methods: the number, shape and adhesion of endothelial cells on the inner wall of tissue engineered vessels were observed by silver nitrate staining and fluorescence labeling of actin in endothelial cells. Results and conclusion: after 2 h and 3.0 N/m~2 shear stress, almost all of the endothelial cells on the inner wall of the vessel shed off, but after the addition of 0.2 N/m~2 / 2 of shear stress every 2 h, the vascular endothelial cell layer remained intact. The morphology of the cells formed an obvious axial arrangement and induced the appearance of stress fibers in endothelial cells. The results show that the "acclimation" of flow shear force in gradient can promote the endothelialization of small caliber tissue engineering vessels.
【作者单位】： 冠昊生物科技股份有限公司再生型医用植入器械国家工程实验室;广东省体育科学研究所;
【基金】：广东省重大科技专项(2010A080407005) 国家重点研究计划生物医用材料研发与组织器官修复替代重点专项(2016YFC1103202)~~
【分类号】：R318.08
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