功能性纳米羟基磷灰石与细胞相互作用及其机制
发布时间：2018-08-29 08:20
【摘要】：目的:选取自备的铽掺杂的精氨酸表面修饰的羟基磷灰石(arginine functionalized hydroxyapatite,HAP/Arg)纳米颗粒,研究该纳米颗粒与细胞的相互作用及血管内皮细胞对HAP/Arg纳米颗粒的内吞动力学和内吞入胞的相关机制。方法:选取人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)作为研究模型细胞,利用激光扫描共聚焦显微镜观察HAP/Arg纳米颗粒的细胞摄取,利用细胞流式仪及原子力显微镜测定不同浓度下细胞摄取后的平均荧光强度。结果:铽掺杂的HAP/Arg纳米颗粒被HUVECs摄取后主要存在于细胞质中,而且大部分分布在细胞核区域周围。HAP/Arg纳米颗粒的入胞过程具有时间和浓度依赖性,作用时间以4 h为宜,纳米颗粒的浓度以50 mg/L为最佳。精氨酸修饰的HAP纳米颗粒入胞量明显强于未经精氨酸修饰的HAP纳米颗粒。结论:HAP/Arg纳米颗粒进入HUVECs是一个主动转运的能量依赖的过程,以网格蛋白和小凹蛋白介导的内吞相结合的细胞跨膜转运机制,但以小凹蛋白介导内吞途径为主。
[Abstract]:Objective: to select the terbium doped arginine surface modified hydroxyapatite (arginine functionalized hydroxyapatite,HAP/Arg) nanoparticles. The interaction between the nanoparticles and cells and the endocytosis kinetics of vascular endothelial cells to HAP/Arg nanoparticles and the mechanism of endocytosis were studied. Methods: human umbilical vein endothelial cells (human umbilical vein endothelial cells,HUVECs) were selected as model cells. The uptake of HAP/Arg nanoparticles was observed by laser scanning confocal microscopy. Cell flow cytometry and atomic force microscope (AFM) were used to measure the average fluorescence intensity of cells at different concentrations. Results: the terbium doped HAP/Arg nanoparticles mainly existed in the cytoplasm after HUVECs uptake, and most of them were located around the nuclear region. The entering process of HAP / Arg nanoparticles was in a time-and concentration-dependent manner, and the appropriate time of action was 4 h. The best concentration of nanoparticles is 50 mg/L. The number of arginine modified HAP nanoparticles was significantly higher than that of unarginine modified HAP nanoparticles. Conclusion the entry of HUVECs nanoparticles into HUVECs is an energy dependent process of active transport, which is mediated by griddle protein and concave protein mediated transmembrane transport mechanism, but mainly by concave protein mediated endocytosis pathway.
【作者单位】： 中南大学湘雅三医院医学实验中心;中南大学医用材料与器械研究中心;
【基金】：湖南省科技厅重点研发计划项目(2016JC2064) 教育部博士学科点基金(20130162120094) 中南大学粉末冶金国家重点实验室开放课题~~
【分类号】：R318.08
本文编号：2210725
[Abstract]:Objective: to select the terbium doped arginine surface modified hydroxyapatite (arginine functionalized hydroxyapatite,HAP/Arg) nanoparticles. The interaction between the nanoparticles and cells and the endocytosis kinetics of vascular endothelial cells to HAP/Arg nanoparticles and the mechanism of endocytosis were studied. Methods: human umbilical vein endothelial cells (human umbilical vein endothelial cells,HUVECs) were selected as model cells. The uptake of HAP/Arg nanoparticles was observed by laser scanning confocal microscopy. Cell flow cytometry and atomic force microscope (AFM) were used to measure the average fluorescence intensity of cells at different concentrations. Results: the terbium doped HAP/Arg nanoparticles mainly existed in the cytoplasm after HUVECs uptake, and most of them were located around the nuclear region. The entering process of HAP / Arg nanoparticles was in a time-and concentration-dependent manner, and the appropriate time of action was 4 h. The best concentration of nanoparticles is 50 mg/L. The number of arginine modified HAP nanoparticles was significantly higher than that of unarginine modified HAP nanoparticles. Conclusion the entry of HUVECs nanoparticles into HUVECs is an energy dependent process of active transport, which is mediated by griddle protein and concave protein mediated transmembrane transport mechanism, but mainly by concave protein mediated endocytosis pathway.
【作者单位】： 中南大学湘雅三医院医学实验中心;中南大学医用材料与器械研究中心;
【基金】：湖南省科技厅重点研发计划项目(2016JC2064) 教育部博士学科点基金(20130162120094) 中南大学粉末冶金国家重点实验室开放课题~~
【分类号】：R318.08
【相似文献】
相关期刊论文 前2条
1 白娟;曹济民;;碳纳米管的跨膜转运、生物学效应及应用[J];医学研究杂志;2008年02期
2 ;[J];;年期
相关会议论文 前1条
1 李月舟;魏华;梅岩艾;周汉清;孙家廷;张志鸿;;物质跨膜转运中机械力的效应影响[A];第九次全国生物物理大会学术会议论文摘要集[C];2002年
，本文编号：2210725
本文链接：https://www.wllwen.com/yixuelunwen/swyx/2210725.html
