光谱法研究聚赖氨酸与纤维蛋白原的相互作用

发布时间：2018-09-09 20:29

【摘要】：聚赖氨酸是一种重要的聚阳离子,在生物医药领域具有广泛的应用前景。但是,目前其血液相容性的相关报道较少,特别是通过光谱法研究其与血液中重要蛋白的相互作用。因此,通过多种光谱法研究聚赖氨酸与纤维蛋白原的相互作用,进一步评价其血液相容性具有一定的创新性。本实验通过荧光、紫外和圆二色谱研究聚赖氨酸对纤维蛋白原结构的影响。其中,聚赖氨酸的正电性随着浓度增大而增大;复合实验显示,0.01mg·mL-1的聚赖氨酸对纤维蛋白原的功能影响较小,随着浓度增大,相互作用增强;荧光光谱显示,纤维蛋白原在λem=341nm处出现浓度依赖性的荧光猝灭;紫外光谱显示,聚赖氨酸对纤维蛋白原吸收强度(200~240和278nm处)的影响在0.025mg·mL-1时较小,并出现浓度依赖性的减少;圆二色光谱显示,随着聚赖氨酸浓度增大,纤维蛋白原的α-螺旋含量减少,β-折叠、β-转角和无规卷曲含量增加。结果表明,聚赖氨酸会与纤维蛋白原发生静电相互作用,对其结构造成浓度依赖性的影响。当浓度为0.01和0.025mg·mL-1时,聚赖氨酸对纤维蛋白原结构的影响较小;而浓度过大时,影响较大,势必破坏纤维蛋白原的生理功能。因此,研发和应用聚赖氨酸时必须充分考虑浓度因素。本实验提供了一种简便而系统的方法来研究材料与蛋白的作用,有利于充分评价材料的血液相容性。此外,上述研究结果对聚赖氨酸的生物医学应用具有重要的指导意义。
[Abstract]:Poly (lysine) is an important polycation, which has a wide application prospect in biomedical field. However, there are few reports about its blood compatibility, especially the study of its interaction with important proteins in blood by spectral method. Therefore, it is innovative to study the interaction between polylysine and fibrinogen by a variety of spectroscopic methods to further evaluate the blood compatibility of polylysine and fibrinogen. The effect of polylysine on the structure of fibrinogen was studied by fluorescence, UV and circular dichroism. The positive electrical properties of polylysine increased with the increase of concentration, and the effect of polylysine (0.01 mg mL-1) on the function of fibrinogen was small, and the interaction increased with the increase of concentration. The fluorescence quenching of fibrinogen in a concentration dependent manner was observed at 位 em=341nm, and the effect of polylysine on the absorptivity of fibrinogen (200 ~ 240 and 278nm) was less than that at 0.025mg mL-1, and decreased in a concentration-dependent manner, the circular dichroism spectrum showed that, The 伪 -helix content of fibrinogen decreased, 尾 -fold, 尾 -rotation angle and random coil content increased with the increase of PLysine concentration. The results showed that polylysine interacted with fibrinogen in a concentration-dependent manner. When the concentration was 0. 01 and 0.025mg mL-1, the effect of polylysine on fibrinogen structure was small, but when the concentration was too high, the physiological function of fibrinogen would be destroyed. Therefore, concentration factors must be fully taken into account in the development and application of Polysine. This experiment provides a simple and systematic method to study the effect of material and protein, which is helpful to evaluate the blood compatibility of the material. In addition, these results are of great significance for the biomedical application of Polysine.
【作者单位】：暨南大学材料科学与工程系;暨南大学附属第一医院;
【基金】：广东省省级科技项目(2014A020215018)资助
【分类号】：R318.08;O657.3

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