组织工程用PLA纤维基支架的制备、成型及细胞相容性研究
发布时间：2018-09-10 07:53
【摘要】：论文基于静电纺丝技术，制备出PLA(polylactic acid)基支架材料：溶液静电纺PLA纤维支架，熔融静电纺PLA纤维支架及溶液静电纺PLA纱线支架。 探讨了溶液静电纺成纤过程中纺丝液射流运动过程：超过临界电压后，纺丝液射流所受电相斥力与表面张力的相互作用，决定着射流从直线区域进入到鞭动区域时刻。而正是射流在鞭动区域的运动引起直径剧减，形成超细纤维。基于此，介入不同成分与组分的溶剂，，以干涉纺丝液射流在运动过程所受的电相斥力对射流在运动过程，特别是鞭动区域的运动构成影响，成功调控制备出直径分布差异的溶液静电纺PLA纤维支架材料及溶液静电纺PLA纱线支架材料。分别借助Fridrikh S V模型与Rutledge G C模型，进一步探讨了不同溶剂成分与组分对溶液静电纺PLA纤维直径差异的调控机制。 借助高速相机在纺丝过程中拍摄的射流照片，探讨了熔融静电纺成纤过程中聚合物射流的运动过程：超过临界电压后，喷丝口处聚合物熔滴从球形被拉伸成锥形，形成泰勒锥，射流向收集端作加速运动；射流运动过程中，表面张力与电相斥力之间的相互竞争作用决定了射流的鞭笞稳定性，进而决定了射流成纤时的细化程度。并基于熔融静电纺丝技术，制备出高度定向排列熔融静电纺PLA纤维支架材料。 采用表面沉积的方法，分别对溶液静电纺PLA纤维支架和熔融静电纺PLA纤维支架进行表面Ti/Cu沉积处理。形貌观察发现：对于溶液静电纺PLA纤维支架，表面Ti/Cu沉积造成了纤维表面凹凸不平的类似多孔结构；而对于熔融静电纺PLA纤维支架，Ti/Cu以纳米级的颗粒沉积在纤维表面的部分区域，并在纤维表面呈岛状生长。接触角测试表明：溶液静电纺制备的PLA纤维支架具有一定的疏水性，而熔融静电纺PLA纤维支架则具有一定的亲水性；表面Ti/Cu沉积使溶液静电纺和熔融静电纺PLA纤维均具有较好的亲水性，特别是表面Ti沉积的熔融静电纺PLA纤维，亲水性大大增加，1s时间内，完全润湿。结合形貌观察结果，探讨Ti/Cu金属颗粒在PLA纤维基表面生长机理发现：在表面Ti/Cu沉积初始阶段，金属颗粒主要以核生长型在静电纺PLA纤维基表面进行生长。 分别针对溶液静电纺PLA纤维支架材料，表面Ti/Cu沉积熔融静电纺PLA纤维支架材料以及静电纺PLA纱线支架材料的细胞相容性进行了研究。MTT (4-甲偶氮唑蓝)检测结果发现：NIH3T3细胞在表面Ti沉积的浸提液中，细胞活力随着培养时间的推移而增大，在72h时甚至超过未处理支架材料；体外毒性评定发现，表面Ti沉积样品，在24h时，具有轻微毒性，而到72h时，毒性逐渐消失至0级。而NIH3T3细胞在表面Cu沉积熔融静电纺PLA纤维支架材料浸提液中，细胞活力随培养时间推移稍有降低。体外毒性评定发现，24h，48h及72h时，Cu沉积熔融静电纺PLA纤维支架材料均表现出轻微毒性。 对不同直径范围的溶液静电纺PLA纤维/纱线支架材料的增殖与粘附结果表明：纤维基支架材料均比浇铸膜支架材料更有利于NIH3T3细胞的粘附和增殖；培养72h后，NIH3T3细胞在所有PLA纤维基支架材料上以不同的增长率进行了增殖；纱线的加捻结构，对细胞在PLA纤维支架材料上的增殖有着较大影响；基于纤维之间孔隙结构差异，孔隙率等因素，无论是在不同直径分布的PLA纤维支架材料，还是纱线支架材料上，难以单独从细胞粘附和增殖，判断纤维直径对细胞生长情况的影响。
[Abstract]:Based on electrospinning technology, PLA (polylactic acid) based scaffolds were prepared: solution electrospinning PLA fiber scaffolds, melt electrospinning PLA fiber scaffolds and solution electrospinning PLA yarn scaffolds.
The process of jet motion in electrospun spinning process was discussed. The interaction between the repulsive force and the surface tension of the jet after exceeding the critical voltage determines the time when the jet enters the whipping region from the linear region. The electrospun PLA fiber scaffolds and the electrospun PLA yarn scaffolds with different diameter distributions were successfully prepared by interfering with the influence of the electro-repulsive force on the motion of the jet, especially in the whipping region, by intervening the solvents with different composition and composition. Drikh S V model and Rutledge G C model were used to investigate the mechanism of different solvent composition on the diameter difference of electrospun PLA fibers.
With the aid of jet photographs taken by high-speed camera during spinning, the movement process of polymer jet in the process of melt electrospinning was discussed. After exceeding the critical voltage, the polymer droplets at the nozzle were stretched from sphere to cone, forming Taylor cone, and the jet flowed to the collector for accelerated motion. Competition between repulsive forces determines the stability of the jet whip and the fineness of the jet fibers. Based on the melt electrospinning technology, the highly oriented melt electrospun PLA fiber scaffolds were prepared.
Surface Ti/Cu deposition was carried out on solution electrospun PLA fiber scaffolds and melt electrospun PLA fiber scaffolds by surface deposition method. Ti/Cu nanoparticles were deposited on part of the fiber surface and grew in island shape on the fiber surface. Contact angle test showed that the PLA fiber scaffolds prepared by solution electrospinning had certain hydrophobicity, while the melt electrospinning PLA fiber scaffolds had certain hydrophilicity; Ti/Cu deposition on the surface made the solution electrospinning and melting static static static. The electrospun PLA fibers have good hydrophilicity, especially the melt electrospun PLA fibers deposited on the surface of Ti. The hydrophilicity of the electrospun PLA fibers is greatly increased, and the PLA fibers are completely wetted within 1 s. Combining with the morphology observation, the growth mechanism of Ti/Cu metal particles on the surface of PLA fibers is discussed. It is found that at the initial stage of Ti/Cu deposition, the metal particles are mainly nucleated. It grows on the surface of electrospinning PLA fiber.
The cell compatibility of solution electrospun PLA fiber scaffolds, melt electrospun PLA fiber scaffolds deposited on Ti/Cu surface and electrospun PLA yarn scaffolds was studied. MTT (4-methazolyl blue) test results showed that the cell viability of NIH3T3 cells in the extract of surface Ti deposition increased with the time of culture. In vitro toxicity assessment showed that Ti deposited on the surface had slight toxicity at 24 h and gradually disappeared to grade 0 at 72 h. However, the cell viability of NIH3T3 cells deposited melt electrospun PLA fiber scaffold extract on the surface of Cu decreased slightly with the passage of culture time. In vitro toxicity assessment showed that Cu deposited electrospun PLA fiber scaffolds exhibited slight toxicity at 24h, 48h and 72h.
The results of proliferation and adhesion of electrospun PLA fiber/yarn scaffolds with different diameters showed that fiber-based scaffolds were more conducive to the adhesion and proliferation of NIH3T3 cells than cast-membrane scaffolds. Twisting structure has a great influence on cell proliferation on PLA fiber scaffolds. Based on the differences of pore structure and porosity between fibers, it is difficult to judge the effect of fiber diameter on cell growth by cell adhesion and proliferation on PLA fiber scaffolds or yarn scaffolds. Influence.
【学位授予单位】：江南大学
【学位级别】：博士
【学位授予年份】：2013
【分类号】：R318.08
本文编号：2233837
[Abstract]:Based on electrospinning technology, PLA (polylactic acid) based scaffolds were prepared: solution electrospinning PLA fiber scaffolds, melt electrospinning PLA fiber scaffolds and solution electrospinning PLA yarn scaffolds.
The process of jet motion in electrospun spinning process was discussed. The interaction between the repulsive force and the surface tension of the jet after exceeding the critical voltage determines the time when the jet enters the whipping region from the linear region. The electrospun PLA fiber scaffolds and the electrospun PLA yarn scaffolds with different diameter distributions were successfully prepared by interfering with the influence of the electro-repulsive force on the motion of the jet, especially in the whipping region, by intervening the solvents with different composition and composition. Drikh S V model and Rutledge G C model were used to investigate the mechanism of different solvent composition on the diameter difference of electrospun PLA fibers.
With the aid of jet photographs taken by high-speed camera during spinning, the movement process of polymer jet in the process of melt electrospinning was discussed. After exceeding the critical voltage, the polymer droplets at the nozzle were stretched from sphere to cone, forming Taylor cone, and the jet flowed to the collector for accelerated motion. Competition between repulsive forces determines the stability of the jet whip and the fineness of the jet fibers. Based on the melt electrospinning technology, the highly oriented melt electrospun PLA fiber scaffolds were prepared.
Surface Ti/Cu deposition was carried out on solution electrospun PLA fiber scaffolds and melt electrospun PLA fiber scaffolds by surface deposition method. Ti/Cu nanoparticles were deposited on part of the fiber surface and grew in island shape on the fiber surface. Contact angle test showed that the PLA fiber scaffolds prepared by solution electrospinning had certain hydrophobicity, while the melt electrospinning PLA fiber scaffolds had certain hydrophilicity; Ti/Cu deposition on the surface made the solution electrospinning and melting static static static. The electrospun PLA fibers have good hydrophilicity, especially the melt electrospun PLA fibers deposited on the surface of Ti. The hydrophilicity of the electrospun PLA fibers is greatly increased, and the PLA fibers are completely wetted within 1 s. Combining with the morphology observation, the growth mechanism of Ti/Cu metal particles on the surface of PLA fibers is discussed. It is found that at the initial stage of Ti/Cu deposition, the metal particles are mainly nucleated. It grows on the surface of electrospinning PLA fiber.
The cell compatibility of solution electrospun PLA fiber scaffolds, melt electrospun PLA fiber scaffolds deposited on Ti/Cu surface and electrospun PLA yarn scaffolds was studied. MTT (4-methazolyl blue) test results showed that the cell viability of NIH3T3 cells in the extract of surface Ti deposition increased with the time of culture. In vitro toxicity assessment showed that Ti deposited on the surface had slight toxicity at 24 h and gradually disappeared to grade 0 at 72 h. However, the cell viability of NIH3T3 cells deposited melt electrospun PLA fiber scaffold extract on the surface of Cu decreased slightly with the passage of culture time. In vitro toxicity assessment showed that Cu deposited electrospun PLA fiber scaffolds exhibited slight toxicity at 24h, 48h and 72h.
The results of proliferation and adhesion of electrospun PLA fiber/yarn scaffolds with different diameters showed that fiber-based scaffolds were more conducive to the adhesion and proliferation of NIH3T3 cells than cast-membrane scaffolds. Twisting structure has a great influence on cell proliferation on PLA fiber scaffolds. Based on the differences of pore structure and porosity between fibers, it is difficult to judge the effect of fiber diameter on cell growth by cell adhesion and proliferation on PLA fiber scaffolds or yarn scaffolds. Influence.
【学位授予单位】：江南大学
【学位级别】：博士
【学位授予年份】：2013
【分类号】：R318.08
【参考文献】
相关博士学位论文 前1条
1 徐佳;纤维组织工程支架的制备及性能研究[D];吉林大学;2009年
本文编号：2233837
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