原花青素在酸性条件下交联制备心脏瓣膜的性能研究

发布时间：2018-09-16 19:28

【摘要】：为了明确原花青素(procyanidins,PC)在酸性条件下交联脱细胞心脏瓣膜是否能够维持其柔顺度、抑制其钙化成核和具备良好的生物学特性,以1 mg/mL PC在酸性条件下(pH3.0~7.4)交联脱细胞心脏瓣膜,采用万能力学测试仪测试研究其柔顺度,并用体外钙化、体外酶降解、细胞黏附、抗凝血和血小板黏附实验研究其抗钙化和相关生物学特性.结果显示,弱酸性条件下交联的脱细胞瓣膜(PC1-pH6.0)呈现出近似于未交联脱细胞瓣膜的柔软程度,交联后拉伸强度显著提高(5.95±0.6)MPa vs(4.64±0.81)MPa,同时弹性模量与未交联对照组相当,显示出较好的柔顺度;PC1-pH6.0组体外可有效抑制瓣膜钙化;PC交联能保护脱细胞瓣膜不被II型胶原酶降解,瓣膜间质细胞在交联脱细胞瓣膜表面黏附良好,交联后的脱细胞瓣膜具有更好的抗血栓潜能.因此,pH6.0条件下PC交联的脱细胞心脏瓣膜具有类似于未交联脱细胞瓣膜的柔顺性,能有效抑制钙化,具有抗酶降解、抗血栓和良好的细胞相容性,有望用作可再细胞化的生物瓣.
[Abstract]:In order to determine whether proanthocyanidin (procyanidins,PC) can maintain its compliance, inhibit its calcification nucleation and have good biological characteristics, acellular heart valves were crosslinked with 1 mg/mL PC in acidic condition (pH3.0~7.4). Its compliance was studied by universal mechanical tester, and its anti-calcification and related biological characteristics were studied by in vitro calcification, enzyme degradation, cell adhesion, anticoagulant and platelet adhesion experiments. The results showed that the cross-linked acellular valves (PC1-pH6.0) were similar to the uncrosslinked acellular valves in softness under weak acid conditions. The tensile strength of the cross-linked valves was significantly increased by (5.95 卤0.6) MPa vs (4.64 卤0.81) MPa, and the elastic modulus was similar to that of the uncrosslinked control group. The results showed that PC1-pH6.0 group could effectively inhibit calcification and PC crosslinking could protect acellular valves from II type collagenase degradation, and the interstitial cells of valves adhered well on the surface of cross-linked acellular valves. The cross-linked acellular valves have better anti-thrombotic potential. Therefore, the acellular heart valve crosslinked by PC at pH 6.0 has the same flexibility as uncrosslinked acellular valve, can effectively inhibit calcification, has anti-enzyme degradation, anti-thrombus and good cytocompatibility, so it is expected to be used as a bioprosthetic flap.
【作者单位】：华东师范大学生命科学学院;中国科学院上海硅酸盐研究所生物材料与组织工程中心;上海市血液中心上海市输血研究所;
【基金】：国家自然科学基金(31070870)
【分类号】：R318.11

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