基于胆固醇改性壳聚糖材料的蛋白吸附及细胞相容性评价

发布时间：2018-09-17 12:13

【摘要】：目的:提高壳聚糖作为生物材料对细胞的主动响应能力.方法:利用胆固醇对壳聚糖进行仿生修饰.通过QCM-D技术考察了壳聚糖改性前后对于白蛋白吸附行为的变化,AFM技术考察了壳聚糖及其衍生物吸附白蛋白前后的膜表面形貌变化;成纤维细胞3T3评价了细胞在不同材料表面的增殖及形态.结果:经胆固醇修饰后的壳聚糖膜表面蛋白吸附能力有显著提升,改性程度越高,白蛋白的吸附能力越强.吸附蛋白前胆固醇-g-壳聚糖膜表面粗糙度随着改性程度增大而升高,而后下降.吸附蛋白后,膜表面粗糙度反而下降,但高改性程度的膜表面却升高.结论:细胞实验结果表明,胆固醇改性后的壳聚糖衍生物具有较好的细胞相容性,且改性后的胆固醇-g-壳聚糖材料在不同程度上具有促进细胞黏附与增殖的能力.
[Abstract]:Objective: to improve the active response of chitosan as a biomaterial to cells. Methods: chitosan was modified by cholesterol. The changes of albumin adsorption behavior before and after chitosan modification were investigated by QCM-D. The surface morphology of chitosan and its derivatives before and after albumin adsorption were investigated. The proliferation and morphology of fibroblasts on different materials were evaluated by 3T3. Results: the adsorption ability of chitosan membrane surface protein was significantly improved after cholesterol modification, and the higher the degree of modification, the stronger the adsorption ability of albumin. The surface roughness of adsorption protein precholesterol-g-chitosan membrane increased with the increase of modification degree, and then decreased. The surface roughness of the membrane decreased after the adsorption of protein, but the surface of the membrane with high degree of modification increased. Conclusion: the results of cell experiments show that the modified chitosan derivatives have good cytocompatibility and the modified cholesterol-g- chitosan materials have the ability to promote cell adhesion and proliferation in varying degrees.
【作者单位】：暨南大学理工学院材料科学与工程系;人工器官与材料教育部工程中心;
【基金】：国家自然科学基金资助项目(31400824;81171459) 广东省科技计划项目(2014A010105030)
【分类号】：R318.08

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