湿性抑菌水凝胶敷料的性能与应用
发布时间：2018-10-08 19:49
【摘要】：目的采用抗菌肽(GBB)替代传统抗菌剂,制备新型湿性抑菌水凝胶敷料。方法将聚丙烯酸钠固定于无纺布上作为抑菌敷料的基底,并用涂抹方式在基底表面吸附上0.004 mg/cm2~1.00 mg/cm2不同浓度的抗菌肽。参照标准YY/T0471.1-2004中的方法测定敷料的吸水率、含水率及水蒸气透过率;采用2015版《中国药典》通则0952黏附力测定第一法,测定初粘力表征材料理化性能;同时采用接触法及抑菌圈法检测敷料抑菌性,评价抑菌敷料抑菌作用大小,并与商业化含银敷料抑菌率进行比较;用噻唑蓝(MTT)法评估检测材料有无明显细胞毒性。结果敷料吸水率平均值可达404.59%,含水率平均值可达80.09%,水蒸气透过率平均值可达1476.67g/m~2/24h,能黏附最大钢球质量10.10g,敷料表面GBB含0.20 mg/cm~2,对大肠埃希菌抑菌率为100%,MTT法检测材料无明显毒性。结论材料基本满足湿性抑菌水凝胶敷料要求,具有优良生物相容性,故有望作为抗感染的创面敷料。
[Abstract]:Objective to prepare a new hydrogel dressing with antimicrobial peptide (GBB) instead of traditional antimicrobial agent. Methods Sodium polyacrylate was immobilized on the non-woven fabric as the substrate of bacteriostasis dressing, and 0.004 mg/cm2~1.00 mg/cm2 antimicrobial peptides were adsorbed on the substrate surface by smear method. The water absorption, moisture content and water vapor transmittance of the dressing were determined by referring to the method in standard YY/T0471.1-2004, the first method was used to determine the adhesion force of the dressing under the general rule 0952 of Chinese Pharmacopoeia, and the physical and chemical properties of the material were characterized by the determination of the initial adhesion. At the same time, contact method and bacteriostatic circle method were used to test the bacteriostasis of the dressing, to evaluate the bacteriostatic effect of the antifungal dressing, and to compare the bacteriostatic rate of the antibacterial dressing with commercial silver dressing. The thiazolyl (MTT) method was used to evaluate the cytotoxicity of the material. Results the average water absorption rate of dressing was 404.59, the average water content was 80.09, the average water vapor transmittance was 1476.67 g / m ~ 2 / 24 h, and the maximum steel ball mass could be adhered to 10.10 g. The bacteriostatic rate of GBB on dressing surface was 0.20 mg/cm~2, and the bacteriostatic rate of Escherichia coli was 100%. Conclusion the material can meet the requirements of wettable bacteriostasis hydrogel dressing and has good biocompatibility, so it is expected to be used as an anti-infection wound dressing.
【作者单位】： 浙江省医疗器械检验院;杭州先端生物技术有限公司;
【基金】：浙江省省属科研院所基金资助项目(2017F50029)
【分类号】：R318.08
，
本文编号：2258050
[Abstract]:Objective to prepare a new hydrogel dressing with antimicrobial peptide (GBB) instead of traditional antimicrobial agent. Methods Sodium polyacrylate was immobilized on the non-woven fabric as the substrate of bacteriostasis dressing, and 0.004 mg/cm2~1.00 mg/cm2 antimicrobial peptides were adsorbed on the substrate surface by smear method. The water absorption, moisture content and water vapor transmittance of the dressing were determined by referring to the method in standard YY/T0471.1-2004, the first method was used to determine the adhesion force of the dressing under the general rule 0952 of Chinese Pharmacopoeia, and the physical and chemical properties of the material were characterized by the determination of the initial adhesion. At the same time, contact method and bacteriostatic circle method were used to test the bacteriostasis of the dressing, to evaluate the bacteriostatic effect of the antifungal dressing, and to compare the bacteriostatic rate of the antibacterial dressing with commercial silver dressing. The thiazolyl (MTT) method was used to evaluate the cytotoxicity of the material. Results the average water absorption rate of dressing was 404.59, the average water content was 80.09, the average water vapor transmittance was 1476.67 g / m ~ 2 / 24 h, and the maximum steel ball mass could be adhered to 10.10 g. The bacteriostatic rate of GBB on dressing surface was 0.20 mg/cm~2, and the bacteriostatic rate of Escherichia coli was 100%. Conclusion the material can meet the requirements of wettable bacteriostasis hydrogel dressing and has good biocompatibility, so it is expected to be used as an anti-infection wound dressing.
【作者单位】： 浙江省医疗器械检验院;杭州先端生物技术有限公司;
【基金】：浙江省省属科研院所基金资助项目(2017F50029)
【分类号】：R318.08
，
本文编号：2258050
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