离子浓度和渗透压在生物活性玻璃抗菌中的作用

发布时间：2018-10-10 20:21

【摘要】：生物材料是一类高新技术材料,在人体组织、器官的诊断和修复过程中与天然材料和其它人造材料相比,功能优异。生物材料已被广泛用于治疗临床的各种运动损伤,其开发经历了三个标志性的阶段。硅酸盐生物活性玻璃是第二代生物材料,根据含硅量不同,分为数种,其中45S5最具有代表性。细菌感染是临床各种常见手术中难题之一。在不增加副作用的前提下,如果生物活性玻璃本身具有抗菌性,生物材料在临床应用中将会有较大的进展。专家致力于硅酸盐生物活性玻璃的抗菌研究和应用,取得了有意义的突破,证实了其抗菌特性。文献报导影响生物活性玻璃抗菌的可能因素有液体环境的pH值、粉体颗粒粒径、粉体对细菌的粘附、液体环境的离子浓度和渗透压等,但离子浓度和渗透压的因素尚未得到进一步证实。本文将液体环境中离子浓度和渗透压作为研究指标,探讨离子浓度和渗透压在硅酸盐生物活性玻璃抗菌中的作用。方法：测定45S5粉体不同浓度和不同浸提时间的浸提液渗透压,根据45S5渗透压范围配制更大范围渗透压的中性溶液,细菌培养后分析数据得出结论。测定粉体浸提液的钠钙浓度,配制更大范围离子浓度的中性溶液,细菌培养后分析数据得出结论。结果：1、45S5粉体浸提液的渗透压值为420-476mosm/kg.不同浸提时间对于渗透压的影响没有显著性影响,不同粉体浓度对渗透压有显著性影响；2、渗透压在200-600mosm/kg范围的中性溶液内,细菌生长无明显变化；3、Na+浓度为4.41-12.34mg/ml,Ca2+浓度为0.009-0.281mg/ml范围的中性溶液内,细菌生长无明显变化。结论：1、生物活性玻璃粉体浸提液的渗透压和粉体浓度成正相关,与浸提时间无明显相关性。2、细菌在200-600mosm/kg的液体环境中生长无明显变化,即此范围内渗透压在生物活性玻璃抗菌中无明显作用3、细菌在Na+浓度为4.41-12.34mg/ml,Ca2+浓度为0.009-0.281mg/ml的液体环境中生长无明显变化仍能存活,即此范围内Na+和Ca2+浓度在生物活性玻璃抗菌中无明显作用。
[Abstract]:Biomaterials are a kind of high-tech materials, which have excellent function compared with natural materials and other artificial materials in the diagnosis and repair of human tissues and organs. Biomaterials have been widely used in the treatment of various clinical sports injuries, its development has undergone three iconic stages. Silicate biologically active glass is the second generation biomaterial. It can be divided into several kinds according to the content of silicon, among which 45S5 is the most representative. Bacterial infection is one of the common surgical problems in clinic. On the premise of no side effects, if the bioactive glass itself has antibacterial properties, biomaterials will make great progress in clinical application. Experts devoted themselves to the antibacterial research and application of silicate biologically active glass. The possible factors affecting the antibacterial activity of bioactive glass are pH value of liquid environment, particle size, adhesion of powder to bacteria, ionic concentration and osmotic pressure of liquid environment, etc. However, the factors of ion concentration and osmotic pressure have not been further confirmed. In this paper, ionic concentration and osmotic pressure in liquid environment are taken as the research index, and the effect of ion concentration and osmotic pressure on antibacterial activity of silicate bioactive glass is discussed. Methods: the osmotic pressure of 45S5 powder was determined with different concentration and time. The neutral solution with larger osmotic pressure was prepared according to the range of 45S5 osmotic pressure. The concentration of sodium and calcium in the extract of powder was determined, and the neutral solution with larger concentration of ions was prepared. The data of bacteria culture were analyzed and the conclusion was drawn. Results: 1the osmotic pressure of the extract was 420-476mosm / kg. Different extraction time had no significant effect on osmotic pressure, but different concentration of powder had significant effect on osmotic pressure, 2, osmotic pressure in neutral solution of 200-600mosm/kg had no obvious change. In neutral solution with the concentration of 4.41 ~ 12.34 mg / ml Ca ~ (2 +) in the range of 0.009-0.281mg/ml, there was no significant change in bacterial growth. Conclusion: 1. The osmotic pressure of the bioactive glass powder extract was positively correlated with the concentration of the powder, but had no significant correlation with the extraction time. 2. There was no obvious change in the growth of bacteria in the liquid environment of 200-600mosm/kg. In this range, the osmotic pressure had no obvious effect on the antibacterial activity of bioactive glass. The bacteria could survive in the liquid environment where the concentration of Na was 4.41 ~ 12.34 mg / ml / ml Ca ~ (2 +) and the concentration of Ca ~ (2 +) was 0.009-0.281mg/ml. In this range, the concentration of Na and Ca2 had no obvious effect on the antibacterial activity of bioactive glass.
【学位授予单位】：华中师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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