电解蚀刻法处理的钛及钛合金表面的对比研究

发布时间：2018-10-13 08:30

【摘要】：目的通过成骨细胞的体外培养,初步探讨钛及钛合金微-纳米三维形貌对成骨细胞生物学行为的影响。方法采用电解蚀刻法在纯钛及钛合金表面构建出不同尺寸的微-纳米三维形貌,并观察其三维结构表面对成骨细胞黏附、增殖、细胞形态、碱性磷酸酶(ALP)活性的影响。结果在成骨细胞的黏附和增殖方面,纯钛组和钛合金组表面均高于纯钛机械抛光组。纯钛组表面细胞胞体饱满,伸出大量伪足,并可见大量功能颗粒。ALP活性显著高于钛合金和纯钛机械抛光组表面。结论通过电解蚀刻法在纯钛和钛合金表面可形成不同直径和深度的碗形巢样及纳米结构;两个表面即30~50μm和5~8μm的表面和光滑表面相比,都明显促进了细胞的附着;30~50μm的纯钛表面更有利于促进细胞的增殖和分化。
[Abstract]:Objective to investigate the effect of titanium and titanium alloy micromorphology on the biological behavior of osteoblasts in vitro. Methods three dimensional microstructures were constructed on the surface of pure titanium and titanium alloy by electrolytic etching, and the effects of three dimensional structure on the adhesion, proliferation, cell morphology and alkaline phosphatase (ALP) activity of osteoblasts were observed. Results in the aspect of osteoblast adhesion and proliferation, the surface of pure titanium group and titanium alloy group were higher than that of pure titanium mechanical polishing group. In pure titanium group, the cell body was full, a large number of pseudopodia were extended, and a large number of functional particles could be seen. The activity of ALP was significantly higher than that of titanium alloy and pure titanium mechanically polished group. Conclusion different diameters and depths of cut-shaped nests and nanostructures can be formed on pure titanium and titanium alloy surfaces by electrolytic etching, and the two surfaces, 30 ~ 50 渭 m and 5 ~ 8 渭 m, are compared with smooth surfaces. The surface of pure titanium at 30 渭 m was more favorable to the proliferation and differentiation of the cells.
【作者单位】：德州市人民医院口腔科;吉林大学口腔医院口腔种植中心;滨州医学院附属医院口腔科;
【基金】：吉林省科技厅基金资助项目(200905175)
【分类号】：R783.1

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