壳聚糖基可注射纳米复合凝胶的制备及表征

发布时间：2018-10-23 18:07

【摘要】：传统的水凝胶支架面临伤口创伤、感染以及对缺损部位不适应等问题,从而导致支架的失效。可注射温敏水凝胶在注射前为流动的液体,通过注射器注入皮下组织或肌肉组织后,可在注射部位原位凝胶化,具有微创易治疗、可修复深层组织损伤、不破坏修复区供血等优点。通常血管内皮生长因子(VEGF)和基质细胞衍生因子-1(SDF-1)的半衰期较短,若将其装载到纳米颗粒复合凝胶中则可起缓释效果。因此实验制备了壳聚糖基水凝胶,保持壳聚糖和明胶体积比不变,通过加入不同体积的β-甘油磷酸钠,制备出不同组分的壳聚糖基温敏水凝胶体系,研究了不同凝胶的成胶时间、孔隙率以及细胞毒性;并制备了壳聚糖和岩藻聚糖的纳米颗粒,对VEGF和SDF-1进行了装载,并对纳米颗粒的粒径、电位及因子释放做了检测。结果表明壳聚糖基水凝胶具有温敏性且增加β-甘油磷酸钠的量会降低凝胶时间,凝胶电镜观察呈三维网络状,无细胞毒性;纳米颗粒粒径分布较均匀且稳定,在凝胶中有缓释效果。因此,制备安全可靠、有合适响应速度的壳聚糖基纳米复合水凝胶是提高材料性能的一种有效方式,将为今后满足不同需求的医用生物材料提供新的研究方向。
[Abstract]:Traditional hydrogel scaffolds face wound trauma, infection and failure to adapt to the defect, which leads to the failure of the scaffold. Injectable thermo-sensitive hydrogel is a fluid flowing before injection. After injecting subcutaneous tissue or muscle tissue through syringe, it can be gelled in situ at the injection site, which is minimally invasive and easy to treat, and can repair deep tissue damage. Do not destroy the repair area of blood supply and other advantages. The half-life of vascular endothelial growth factor (VEGF) and stromal cell derived factor-1 (SDF-1) is usually shorter, but it can be released slowly if loaded into nano-particle composite gel. Therefore, chitosan based hydrogels were prepared, which kept the volume ratio of chitosan to gelatin unchanged. By adding different volumes of 尾 -glycerophosphate, different components of chitosan based thermo-sensitive hydrogels were prepared. The gelation time, porosity and cytotoxicity of different gels were studied, and the nanoparticles of chitosan and fucosan were prepared, VEGF and SDF-1 were loaded, and the particle size, potential and factor release were determined. The results showed that chitosan hydrogel had temperature sensitivity and increased the amount of 尾 -glycerophosphate, and the gel time was decreased. The gel electron microscope showed three-dimensional network shape, no cytotoxicity, and the particle size distribution was uniform and stable. It has slow release effect in gel. Therefore, the preparation of chitosan based nanocomposite hydrogels, which is safe and reliable and has a suitable response speed, is an effective way to improve the properties of the materials. It will provide a new research direction for medical biomaterials that meet different requirements in the future.
【作者单位】：西南交通大学生命科学与工程学院;西南交通大学材料先进技术教育部重点实验室;
【基金】：国家自然科学基金资助项目(NSFC81401522) 中央高校基本科研业务费专项资金,医学类专题研究资助项目(2682016YXZT15)
【分类号】：R318.08

【相似文献】