硅磷酸钙骨水泥的理化性质及其生物相容性研究
发布时间：2018-11-02 15:17
【摘要】：实验目的：复合C3S和磷酸钙系(磷酸氢钙DCPA+磷酸四钙TTCP)骨水泥，以期能改善骨水泥的降解性，提高其抗压强度和力学性能，调节固化时间，提高骨水泥的生物相容性。 实验方法：(1)用吉尔摩(Gilmore)双针法测定复合骨水泥的固化时间；在Instron5567型万能材料实验机上测试抗压强度；模拟体液(SBF)中浸泡后，骨水泥浸提液的pH值及其降解率随时间的变化，并结合SEM和XRD微观结构的观察，对骨水泥理化性质的动态变化规律进行描述；(2)MTT实验结合实时定量研究复合骨水泥的生物相容性。 实验结果：(1)随着C3S百分含量的增大，固化时间先增加后减少然后又增加。(2)随着C3S百分含量的增加，骨水泥的抗压强度先升高后降低，在C3S的含量为40%时，骨水泥的抗压强度达到最大值21.6Mpa，比磷酸钙系(TTCP+DCPA)骨水泥的强度稍高。(3)复合骨水泥具有较好降解性，而且随着C3S百分含量的增加，骨水泥的降解率增大，在C3S的含量为40%时，骨水泥的降解率达到最大值10.26%，浸泡完成后复合骨水泥维持较高的抗压强度，在浸泡到第7d时，抗压强度达到最大值27.84MPa。复合骨水泥浸泡7d后浸泡液的pH值接近中性。(4)添加一定量的C3S骨水泥能刺激细胞的增殖，并提高CyclinD1、PCNA、SDH基因的表达。 实验结论：C3S的引入延长了磷酸钙系(TTCP+DCPA)骨水泥的固化时间，提高了骨水泥的降解性，在一定程度上提高了磷酸钙系骨水泥的抗压强度。制备的复合骨水泥克服了磷酸钙系(TTCP+DCPA)骨水泥降解率低的缺点，，而且具有适宜的固化时间，较高的抗压强度和良好的生物相容性和安全性，可作为一种潜在的骨水泥修复材料。
[Abstract]:Objective: to combine C3s with calcium phosphate (DCPA) tetracalcium phosphate (TTCP) bone cement in order to improve the degradation of bone cement, improve its compressive strength and mechanical properties, adjust the curing time, and improve the biocompatibility of bone cement. Methods: (1) the curing time of composite bone cement was determined by Gilmore (Gilmore) double needle method, and the compressive strength was measured on Instron5567 universal material experiment machine. After soaking in simulated body fluid (SBF), the pH value and degradation rate of bone cement extract changed with time. The dynamic changes of physical and chemical properties of bone cement were described by observing the microstructure of SEM and XRD. (2) the biocompatibility of composite bone cement was studied by MTT experiment and real time quantitative analysis. The results were as follows: (1) with the increase of C3s content, the curing time increased first, then decreased, and then increased. (2) with the increase of C3S content, the compressive strength of bone cement increased first and then decreased, when the content of C3S was 40%, The compressive strength of bone cement reached the maximum value of 21.6 Mpaa, which was slightly higher than that of calcium phosphate (TTCP DCPA) cement. (3) the composite bone cement had better degradability, and the degradation rate of bone cement increased with the increase of C3s content. The degradation rate of bone cement reached the maximum value of 10.26 when the content of C _ 3s was 40 and the compressive strength of composite bone cement was maintained after immersion. The compressive strength reached the maximum of 27.84 MPA at the 7th day after immersion. (4) adding a certain amount of C3s bone cement could stimulate cell proliferation and increase the expression of CyclinD1,PCNA,SDH gene. Conclusion: the introduction of C3S can prolong the curing time of calcium phosphate (TTCP DCPA) cement, improve the degradation of bone cement, and improve the compressive strength of calcium phosphate bone cement to some extent. The prepared composite bone cement overcomes the shortcoming of low degradation rate of calcium phosphate (TTCP DCPA) cement, and has the advantages of suitable curing time, high compressive strength, good biocompatibility and safety. It can be used as a potential bone cement repair material.
【学位授予单位】：河南师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08
[Abstract]:Objective: to combine C3s with calcium phosphate (DCPA) tetracalcium phosphate (TTCP) bone cement in order to improve the degradation of bone cement, improve its compressive strength and mechanical properties, adjust the curing time, and improve the biocompatibility of bone cement. Methods: (1) the curing time of composite bone cement was determined by Gilmore (Gilmore) double needle method, and the compressive strength was measured on Instron5567 universal material experiment machine. After soaking in simulated body fluid (SBF), the pH value and degradation rate of bone cement extract changed with time. The dynamic changes of physical and chemical properties of bone cement were described by observing the microstructure of SEM and XRD. (2) the biocompatibility of composite bone cement was studied by MTT experiment and real time quantitative analysis. The results were as follows: (1) with the increase of C3s content, the curing time increased first, then decreased, and then increased. (2) with the increase of C3S content, the compressive strength of bone cement increased first and then decreased, when the content of C3S was 40%, The compressive strength of bone cement reached the maximum value of 21.6 Mpaa, which was slightly higher than that of calcium phosphate (TTCP DCPA) cement. (3) the composite bone cement had better degradability, and the degradation rate of bone cement increased with the increase of C3s content. The degradation rate of bone cement reached the maximum value of 10.26 when the content of C _ 3s was 40 and the compressive strength of composite bone cement was maintained after immersion. The compressive strength reached the maximum of 27.84 MPA at the 7th day after immersion. (4) adding a certain amount of C3s bone cement could stimulate cell proliferation and increase the expression of CyclinD1,PCNA,SDH gene. Conclusion: the introduction of C3S can prolong the curing time of calcium phosphate (TTCP DCPA) cement, improve the degradation of bone cement, and improve the compressive strength of calcium phosphate bone cement to some extent. The prepared composite bone cement overcomes the shortcoming of low degradation rate of calcium phosphate (TTCP DCPA) cement, and has the advantages of suitable curing time, high compressive strength, good biocompatibility and safety. It can be used as a potential bone cement repair material.
【学位授予单位】：河南师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08
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