氧化锆陶瓷材料生物相容性的实验研究
发布时间：2018-11-06 20:05
【摘要】：目的 评估用于牙种植基台的注射成型和特殊烧结工艺下氧化锆陶瓷材料在生物学方面的相容性,为口腔种植及其他修复的临床应用提供实验依据。 方法 实验一.用注射成型和特殊烧结工艺下氧化锆全瓷材料试件制作的的浸提液,分别进行四氮唑盐比色法(MTT)体外细胞毒性试验和溶血试验。 实验二.对Wister大鼠的口腔黏膜刺激实验：将新型氧化锆陶瓷圆片状试件缝合、固定在实验大鼠的左侧颊黏膜表面,使其与黏膜紧密贴合但无压迫,术后2周,将实验大鼠处死进行研究,应用大体观察和组织学方法,宏观微观结合观察大鼠口腔颊黏膜与注射成型和特殊烧结工艺下,规范片状的氧化锆陶瓷材料的反应；选用与氧化锆试件规格相同的牙胶缝合于右侧颊黏膜做对照。 结果 实验一.氧化锆全瓷材料组织相容性的细胞毒性评分小于Ⅰ级,溶血率为2.14%(P0.05),无急性毒性反应,无溶血反应。 实验二.口腔黏膜刺激实验：实验动物氧化锆陶瓷试件周围的颊黏膜均无充血、肿胀、变性、糜烂及溃疡等病理变化,组织学观察无炎细胞浸润,未见异物细胞；在统计学上,与实验设置的对照组无显著性的差异 结论 注射成型和特殊烧结工艺下氧化锆陶瓷材料具有良好的组织相容性,生物安全性,对口腔黏膜有良好的生物相容性。
[Abstract]:Objective to evaluate the biological compatibility of zirconia ceramic materials used in dental implant abutment injection molding and special sintering process, and to provide experimental evidence for the clinical application of dental implant and other restorations. Methods experiment 1. In vitro cytotoxicity test and hemolysis test of (MTT) with tetrazolium salt colorimetry were carried out in the extraction solution of zirconia all-ceramic material made by injection molding and special sintering process. Experiment two The oral mucosal stimulation test of Wister rats: the new zirconia ceramic flake specimens were sutured and fixed on the left buccal mucosa surface of the experimental rats, so that they were tightly attached to the mucosa without compression. The experimental rats were killed 2 weeks after the operation. The reaction of the buccal mucosa of the rat with injection molding and special sintering process was observed by gross observation and histology, macroscopically and microscopically, in order to standardize the flake zirconia ceramic material. The right buccal mucosa was sutured to the right buccal mucosa with the same size as the zirconia specimen. Results experiment 1. The cytotoxicity score of Zirconia all-ceramic material was lower than that of grade 鈪，
本文编号：2315330
[Abstract]:Objective to evaluate the biological compatibility of zirconia ceramic materials used in dental implant abutment injection molding and special sintering process, and to provide experimental evidence for the clinical application of dental implant and other restorations. Methods experiment 1. In vitro cytotoxicity test and hemolysis test of (MTT) with tetrazolium salt colorimetry were carried out in the extraction solution of zirconia all-ceramic material made by injection molding and special sintering process. Experiment two The oral mucosal stimulation test of Wister rats: the new zirconia ceramic flake specimens were sutured and fixed on the left buccal mucosa surface of the experimental rats, so that they were tightly attached to the mucosa without compression. The experimental rats were killed 2 weeks after the operation. The reaction of the buccal mucosa of the rat with injection molding and special sintering process was observed by gross observation and histology, macroscopically and microscopically, in order to standardize the flake zirconia ceramic material. The right buccal mucosa was sutured to the right buccal mucosa with the same size as the zirconia specimen. Results experiment 1. The cytotoxicity score of Zirconia all-ceramic material was lower than that of grade 鈪，
本文编号：2315330
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