一种新型巴沙鱼皮胶原支架材料的理化性能及体外降解性
发布时间：2018-11-08 09:44
【摘要】：目的以巴沙鱼皮为原料制备新型胶原支架材料,并对该材料的结构特性、物理性质以及体外降解性进行检测和分析,以探索巴沙鱼是否可以取代陆生哺乳动物作为胶原支架材料的合适来源。方法巴沙鱼皮经过反复脱脂、脱色、脱杂处理后,冷冻干燥成膜状。采用凯氏定氮法测定粗蛋白含量,扫描电镜观察和分析材料的结构以及孔隙的大小和分布,液体置换法测孔隙率,万能试验机检测抗拉强度。观察材料的黏度随温度的变化情况,以检测材料的变性温度。将材料浸泡在置于37℃恒温震荡箱的磷酸盐缓冲液(0.1mol/L,pH 7.4)中,检测材料的吸水率和失重率。结果材料的粗蛋白含量为95.2%。肉眼观察厚薄均匀。扫描电镜显示材料的一面较为粗糙,可见多孔结构,孔径大小不一,分布均匀;另一面光滑,孔隙致密。孔隙率(55.50±1.94)%,厚度(0.66±0.10)mm,抗拉强度(18.82±0.94)MPa。未经交联的材料变性温度为34℃,经热交联后为36℃。48h吸水率(379.77±77.81)%。经热交联后的材料28d的失重率为(80.22±2.49)%,缓冲液pH为6.67±0.05。结论巴沙鱼皮胶原支架材料可制成厚薄均匀、具有致密层和疏松层双层不同结构的生物膜。材料具有良好的机械强度、较为适宜的变性温度,但降解速率较快,有待改进。
[Abstract]:Objective to prepare a new collagen scaffold material from the Barsa skin, and to detect and analyze its structure, physical properties and biodegradability in vitro. To explore whether the fish can replace terrestrial mammals as a suitable source of collagen scaffolds. Methods after repeatedly degreasing, decolorizing and removing impurity, the skin was freeze-dried to form a film. The crude protein content was determined by Kjeldahl nitrogen determination method, the structure and pore size and distribution of the material were observed and analyzed by SEM, the porosity was measured by liquid displacement method, and the tensile strength was measured by universal testing machine. Observe the change of the viscosity with temperature to detect the denaturation temperature of the material. The material was immersed in phosphate buffer solution (0.1 mol / L 路L ~ (-1) pH 7.4) in a 37 鈩，
本文编号：2318105
[Abstract]:Objective to prepare a new collagen scaffold material from the Barsa skin, and to detect and analyze its structure, physical properties and biodegradability in vitro. To explore whether the fish can replace terrestrial mammals as a suitable source of collagen scaffolds. Methods after repeatedly degreasing, decolorizing and removing impurity, the skin was freeze-dried to form a film. The crude protein content was determined by Kjeldahl nitrogen determination method, the structure and pore size and distribution of the material were observed and analyzed by SEM, the porosity was measured by liquid displacement method, and the tensile strength was measured by universal testing machine. Observe the change of the viscosity with temperature to detect the denaturation temperature of the material. The material was immersed in phosphate buffer solution (0.1 mol / L 路L ~ (-1) pH 7.4) in a 37 鈩，
本文编号：2318105
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