脱钙松质骨体外构建组织工程软骨的实验研究
发布时间：2018-11-11 18:26
【摘要】：目的软骨细胞复合同种异体脱钙松质骨(demineralized cancellous bone,DCB)体外构建组织工程软骨,评价DCB作为组织工程软骨支架的可行性。方法取新西兰兔长骨干骺端松质骨制备DCB,扫描电镜观察;分离幼兔关节软骨细胞,原代培养后种植在同种异体DCB体外培养,分别于7、14、21、28、35、42d取材进行苏木素-伊红(HE)、甲苯胺蓝(TB)、Ⅰ型和Ⅱ型胶原染色。结果制备的DCB成三维立体多孔结构,孔隙大小100~500μm,有良好的可塑性和一定的力学强度;软骨细胞在DCB表面和空隙内生长、分化良好,形成软骨陷窝,分泌基质蛋白聚糖和Ⅱ型胶原。培养42d软骨细胞在DCB表面及孔隙内形成软骨样组织。结论 DCB复合同种异体软骨细胞在体外成功构建了组织工程软骨,是一种较理想的软骨组织工程支架材料。
[Abstract]:Objective to construct tissue engineered cartilage with chondrocytes combined with allogeneic decalcified cancellous bone (demineralized cancellous bone,DCB) in vitro and evaluate the feasibility of DCB as scaffold for tissue engineering cartilage. Methods New Zealand rabbit epiphyseal cancellous bone was prepared by DCB, scanning electron microscope. Articular chondrocytes were isolated from young rabbits and cultured in vitro with allogeneic DCB. The specimens were collected for (TB), 鈪，
本文编号：2325744
[Abstract]:Objective to construct tissue engineered cartilage with chondrocytes combined with allogeneic decalcified cancellous bone (demineralized cancellous bone,DCB) in vitro and evaluate the feasibility of DCB as scaffold for tissue engineering cartilage. Methods New Zealand rabbit epiphyseal cancellous bone was prepared by DCB, scanning electron microscope. Articular chondrocytes were isolated from young rabbits and cultured in vitro with allogeneic DCB. The specimens were collected for (TB), 鈪，
本文编号：2325744
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