具有NO原位催化能力和内皮细胞特异性粘附的小口径血管支架的制备和表征

发布时间：2018-11-12 11:37

【摘要】：人工合成的小口径血管支架亟需攻克抗凝血和快速内皮化两大难题。负载有机硒催化剂聚乙烯亚胺(SePEI)能够催化一氧化氮(NO)的原位生成,从而有效地抑制血栓形成。层粘连蛋白来源多肽Tyr-Ile-Gly-Ser-Arg(YIGSR)具有内皮细胞选择性粘附功能,负载到支架表面能够诱导内皮层的快速形成。本实验采用静电纺丝构建聚己内酯(PCL)支架,通过层层自组装构建Se PEI和透明质酸(HA)的双电层催化NO的生成;最外层的HA接枝有YIGSR多肽促进内皮化。考察和比较了材料修饰前后的物化性能。修饰过程对纤维形貌无明显影响,负载稳定性经过65d仍能保持在99%以上。材料经修饰后力学性能有所提高,能够满足作为血管支架的要求,为下一步细胞培养和体内实验打下基础。
[Abstract]:Synthetic small-caliber vascular stents need to overcome both anticoagulant and rapid endothelial problems. Polyethyleneimine (SePEI) supported on organic selenium catalyst can catalyze the in situ formation of nitric oxide (NO), thus effectively inhibiting thrombosis. Laminin derived polypeptide (Tyr-Ile-Gly-Ser-Arg (YIGSR) has the function of selective adhesion of endothelial cells, and loading onto the scaffold surface can induce the rapid formation of endodermis. In this experiment, polycaprolactone (PCL) scaffolds were constructed by electrospinning, and the formation of NO was catalyzed by layers of self-assembly of Se PEI and hyaluronic acid (HA). The outermost HA grafted with YIGSR polypeptide promoted endothelization. The physical and chemical properties of the materials before and after modification were investigated and compared. The modification process had no significant effect on the morphology of the fibers, and the stability of the fibers remained above 99% after 65 days. The modified materials can meet the requirements of vascular scaffold and lay the foundation for cell culture and in vivo experiments.
【作者单位】：南开大学药物化学生物学国家重点实验室生命科学学院生物活性材料教育部重点实验室;南开大学化学学院功能高分子材料教育部重点实验室;
【基金】：国家重大专项(2017YFC 1103504) 国家自然科学基金面上项目(81671842) 天津市自然科学基金重点项目(16JCZDJC37600);PCSIRT(No.IRT13023) 药物化学生物学国家重点实验室开放基金(201603007)
【分类号】：R318.1;TQ340.64

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