纯钛钛片表面不同生物大分子涂层的比较研究

发布时间：2018-11-13 13:01

【摘要】：目的探讨如何在纯钛钛片表面构建不同生物大分子涂层,以期找到合适的种植体表面涂层。方法纯钛钛片表面经打磨抛光及氧化性混酸溶液处理(酸蚀组),设为对照,接着通过层层自组装技术将Ⅰ型胶原(COL)、透明质酸(HA)、壳聚糖(CHI)和多聚谷氨酸(PGA)引入到钛片表面(涂层组)。采用扫描电镜(SEM)、X射线光电子能谱(XPS)、石英晶体微量天平(QCM)和接触角试验对钛片的表面进行表征。在各种钛片表面培养成骨前体细胞,检测其早期黏附、增殖及分化的能力。结果SEM结果显示,各组涂层的表面非常相似、平整。XPS分析发现各组钛片表面元素成分氮的含量随着组装层数的增加而不断升高。接触角实验显示在纯钛表面组装生物大分子并没有损害基底面良好的亲水性。涂层组钛片较酸蚀组明显促进了成骨细胞的早期黏附、增殖和分化。胶原组[Ti-(COL/HA)5-COL、Ti-(COL/PGA)5-COL]较壳聚糖组[Ti-(CHI/HA)5-CHI、Ti-(CHI/PGA)5-CHI]具有更高的生物活性。而Ti-(COL/HA)5-COL与Ti-(COL/PGA)5-COL相比更能促进成骨细胞的黏附、增殖和分化。结论生物大分子COL、HA、CHI和PGA能够通过LBL技术被成功地引入到纯钛钛片表面。Ti-(COL/HA)5-COL具有较高的生物活性,可能有利于种植体的骨整合。
[Abstract]:Objective to investigate how to construct different biomolecular coatings on titanium substrate in order to find a suitable implant surface coating. Methods the surface of pure titanium sheet was polished and treated with oxidizing mixed acid solution (acid etching group), and then the type I collagen (COL), hyaluronic acid (HA), was prepared by layer self-assembly technique. Chitosan (CHI) and poly-glutamate (PGA) were introduced into the surface of titanium (coating group). The surface of titanium wafer was characterized by scanning electron microscopy (SEM), X) photoelectron spectroscopy (XPS) (XPS), quartz crystal microbalance (QCM) and contact angle test. Osteoblasts were cultured on the surface of various titanium plates, and the ability of early adhesion, proliferation and differentiation of osteoblasts was tested. Results the results of SEM showed that the surface of each coating was very similar and smooth. XPS analysis showed that the content of nitrogen on the surface of each group of titanium sheets increased with the increase of the number of assembled layers. Contact angle experiments showed that the assembled biomolecules on pure titanium surface did not damage the hydrophilicity of the substrate surface. Compared with acid etching group, the coating group promoted the early adhesion, proliferation and differentiation of osteoblasts. The bioactivity of collagen group [Ti- (COL/HA) 5-COLTi- (COL/PGA) 5-COL] was higher than that of chitosan group [Ti- (CHI/HA) 5-CHITi- (CHI/PGA) 5-CHI]. Compared with Ti- (COL/PGA) 5-COL, Ti- (COL/HA) 5-COL could promote the adhesion, proliferation and differentiation of osteoblasts. Conclusion COL,HA,CHI and PGA can be successfully introduced to the surface of titanium tablets by LBL. Ti- (COL/HA) 5-COL has high bioactivity and may be beneficial to bone integration of implants.
【作者单位】：浙江省台州医院口腔科;
【基金】：浙江省科技厅科研基金(2014C33280)
【分类号】：R783.1

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