丝素蛋白纳米羟基磷灰石复合材料的生物安全性

发布时间：2018-11-17 12:17

【摘要】：背景:丝素蛋白具有良好的生物相容性、生物降解性、优越的力学性能,与纳米羟基磷灰石的复合可以模拟天然骨组织中的有机无机成分,可以弥补单纯纳米羟基磷灰石力学性能的不足。目的:评价丝素蛋白/纳米羟基磷灰石复合材料的生物安全性。方法:以氯化钙和磷酸氢二铵为原料,丝素蛋白作为调控纳米羟基磷灰石晶体生长的模板,通过共沉淀法合成了丝素蛋白/纳米羟基磷灰石类骨质复合材料。按照国际标准化组织(ISO10993)和国内(GB/T16886)对于生物材料相容性的检测方法和要求,通过体外细胞毒性、全身急性毒性实验、溶血实验来评价丝素蛋白/纳米羟基磷灰石复合材料的生物安全性。结果与结论:(1)丝素蛋白/纳米羟基磷灰石复合材料浸提液与L929细胞共培养后对细胞的形态、代谢及增殖无影响;(2)丝素蛋白/纳米羟基磷灰石复合材料浸提液腹腔注射到小白鼠体内,对小白鼠无明显不良反应;(3)丝素蛋白/纳米羟基磷灰石复合材料浸提液导致的静脉血溶血率为2.39%,小于5%的国际标准;(4)结果提示,丝素蛋白/纳米羟基磷灰石复合材料无细胞毒性,无全身急性毒性反应,不引起溶血反应,具有良好的生物安全性。
[Abstract]:Background: silk fibroin has good biocompatibility, biodegradability and excellent mechanical properties. The composite of silk fibroin with nano-hydroxyapatite can simulate the organic and inorganic components in natural bone tissue. It can make up for the shortage of mechanical properties of nano-hydroxyapatite. Objective: to evaluate the biological safety of silk fibroin / nano hydroxyapatite composites. Methods: silk fibroin / nano-hydroxyapatite composite was synthesized by co-precipitation with calcium chloride and diammonium hydrogen phosphate as raw materials and fibroin as template to regulate the growth of nano-hydroxyapatite crystals. According to the methods and requirements of International Organization for Standardization (ISO10993) and International Organization for Standardization (GB/T16886) for the biocompatibility of biomaterials, in vitro cytotoxicity, systemic acute toxicity test, Hemolysis test was used to evaluate the biological safety of silk fibroin / nano hydroxyapatite composites. Results and conclusion: (1) the fibroin / nano-hydroxyapatite composite extract had no effect on the morphology, metabolism and proliferation of L929 cells after co-culture. (2) the fibroin / nano-hydroxyapatite composite extract was injected intraperitoneally into the mice, and there was no obvious adverse reaction to the mice. (3) the venous hemolysis rate of silk fibroin / nano-hydroxyapatite composite extract is 2.39, which is less than 5% international standard; (4) the results showed that fibroin / nano-hydroxyapatite composite had no cytotoxicity, no systemic acute toxicity, no hemolytic reaction and good biological safety.
【作者单位】：解放军第175医院/厦门大学附属东南医院全军骨科中心;解放军第二军医大学长征医院骨科;
【基金】：国家自然科学基金面上项目(81472071) 军队医学科技青年培育计划(15QNP022) 南京军区医学科技创新课题面上项目(15MS109)~~
【分类号】：R318.08

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