丝素纤维人工血管材料表面改性及其性能研究
发布时间：2018-11-18 13:42
【摘要】：家蚕丝素纤维有着良好的韧性、强度和生物可降解性,在生物医用材料领域有着广泛的应用。研究表明,丝素纤维机织管状织物可以作为一种力学性能优良的小口径人工血管材料,然而其生物相容性并不理想。因此,本研究以丝素纤维平纹织物为原材料,采用等离子体处理技术,增加材料表面极性基团的数量,然后使用化学偶联剂1-(3-二甲基氨基丙基)-3-乙基羰二亚胺(EDC2HCl)和N-羟基琥珀酰亚胺(NHS)将再生丝素蛋白接枝到丝素纤维材料表面。期望得到力学性能优异、生物相容性良好的丝素纤维人工血管材料,为丝素纤维人工血管的开发提供参考。本论文的主要内容包括:1、采用低温低压等离子体技术对丝素纤维人工血管材料表面进行处理,探究了处理气体、时间、功率、真空度等对处理效果的影响规律;2、分别将高、低分子量再生丝素蛋白接枝到丝素纤维人工血管材料表面,探究了蛋白分子量和浓度对接枝效果的影响;3、对改性后的丝素纤维人工血管材料进行了蛋白吸附、溶血和细胞毒性实验,对其生物相容性进行了评估。研究结果及主要结论如下:(1)相比于O_2、Ar两种等离子体,He等离子体处理对丝素纤维材料的表面形态、结构和力学性能的影响最小,而且能够显著提高材料表面的亲水性。He等离子体处理的优化工艺为:时间180s、功率70w、真空度50Pa。(2)高分子量再生丝素蛋白接枝后,丝素纤维人工血管材料拉伸强度得到提高,但材料的弹性和亲水性有所下降;低分子量再生丝素蛋白接枝后,丝素纤维人工血管材料的拉伸强度也有所提高,而且接枝均匀性和稳定性较好,弹性和亲水性下降程度小。最终得到的优化接枝浓度为:1%的高分子量再生丝素蛋白溶液和5%低分子量再生丝素蛋白溶液。(3)高分子量再生丝素蛋白接枝后,丝素纤维人工血管材料吸附的血浆白蛋白更多。溶血和细胞毒性实验结果表明,相对于低分子量再生丝素蛋白,高分子量再生丝素蛋白接枝能够显著地提高材料的血液和细胞相容性。综上所述,通过He等离子体处理可以保持丝素纤维人工血管材料表面形态完整,同时力学性能损失小、亲水性提高明显。相对于低分子量再生丝素蛋白而言,接枝高分子量再生丝素蛋白可以更明显的提高丝素纤维人工血管材料的生物相容性,但存在接枝后材料弹性降低等问题,接枝工艺有待于进一步完善。
[Abstract]:Silk fiber has good toughness, strength and biodegradability. It is widely used in biomedical materials. The research shows that silk fiber woven tubular fabric can be used as a small diameter artificial vascular material with excellent mechanical properties, but its biocompatibility is not ideal. Therefore, in this study, silk fiber plain fabric was used as raw material and plasma treatment technology was used to increase the number of polar groups on the surface of the material. Then the regenerated fibroin protein was grafted onto the fibroin fiber surface using the chemical coupling agents 1- (3-dimethyl aminopropyl) -3-ethyl carbonyl diimide (EDC2HCl) and N-hydroxysuccinimide (NHS). It is expected to obtain silk fibroin fiber artificial vascular materials with excellent mechanical properties and good biocompatibility, which can provide reference for the development of silk fiber artificial blood vessels. The main contents of this thesis are as follows: 1. The surface of silk fibroin fiber artificial vascular material was treated by low temperature and low pressure plasma technology, and the effect of gas, time, power, vacuum on the treatment effect was investigated. 2Graft of high and low molecular weight regenerated fibroin onto the surface of fibroin fiber artificial vascular material, and the effects of molecular weight and concentration of protein on grafting effect were investigated. 3. Protein adsorption, hemolysis and cytotoxicity of the modified fibroin fiber artificial vascular materials were studied, and their biocompatibility was evaluated. The results and main conclusions are as follows: (1) compared with the two kinds of plasma, He plasma treatment has the least influence on the surface morphology, structure and mechanical properties of silk fibroin fiber. Moreover, the hydrophilicity of the surface of the material can be improved significantly. The optimized process of He plasma treatment is as follows: time 180 s, power 70 wand, vacuum degree 50 Pa. (2) after high molecular weight regenerated fibroin was grafted, The tensile strength of fibroin fiber artificial vascular material was improved, but the elasticity and hydrophilicity of the material decreased. The tensile strength of fibroin fiber artificial vascular materials was also improved after grafting with low molecular weight regenerated silk fibroin. The grafted fibroin fiber had better homogeneity and stability, and the degree of elasticity and hydrophilicity decreased slightly. The optimized graft concentration was 1% high molecular weight regenerated silk fibroin solution and 5% low molecular weight regenerated silk fibroin solution. (3) High molecular weight regenerated silk fibroin was grafted. Fibroin fiber artificial vascular materials adsorb more plasma albumin. The results of hemolysis and cytotoxicity test showed that compared with low molecular weight regenerated fibroin, the graft of high molecular weight regenerated fibroin could significantly improve the blood and cytocompatibility of the materials. To sum up, the surface morphology of silk fibroin fiber artificial vascular material can be kept intact by He plasma treatment, and the mechanical property loss is small, and the hydrophilicity is improved obviously. Compared with the low molecular weight regenerated fibroin protein, grafted high molecular weight regenerated fibroin protein can obviously improve the biocompatibility of fibroin fiber artificial vascular materials, but there are some problems such as the decrease of material elasticity after grafting. The grafting process needs to be further improved.
【学位授予单位】：东华大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R318.08
本文编号：2340185
[Abstract]:Silk fiber has good toughness, strength and biodegradability. It is widely used in biomedical materials. The research shows that silk fiber woven tubular fabric can be used as a small diameter artificial vascular material with excellent mechanical properties, but its biocompatibility is not ideal. Therefore, in this study, silk fiber plain fabric was used as raw material and plasma treatment technology was used to increase the number of polar groups on the surface of the material. Then the regenerated fibroin protein was grafted onto the fibroin fiber surface using the chemical coupling agents 1- (3-dimethyl aminopropyl) -3-ethyl carbonyl diimide (EDC2HCl) and N-hydroxysuccinimide (NHS). It is expected to obtain silk fibroin fiber artificial vascular materials with excellent mechanical properties and good biocompatibility, which can provide reference for the development of silk fiber artificial blood vessels. The main contents of this thesis are as follows: 1. The surface of silk fibroin fiber artificial vascular material was treated by low temperature and low pressure plasma technology, and the effect of gas, time, power, vacuum on the treatment effect was investigated. 2Graft of high and low molecular weight regenerated fibroin onto the surface of fibroin fiber artificial vascular material, and the effects of molecular weight and concentration of protein on grafting effect were investigated. 3. Protein adsorption, hemolysis and cytotoxicity of the modified fibroin fiber artificial vascular materials were studied, and their biocompatibility was evaluated. The results and main conclusions are as follows: (1) compared with the two kinds of plasma, He plasma treatment has the least influence on the surface morphology, structure and mechanical properties of silk fibroin fiber. Moreover, the hydrophilicity of the surface of the material can be improved significantly. The optimized process of He plasma treatment is as follows: time 180 s, power 70 wand, vacuum degree 50 Pa. (2) after high molecular weight regenerated fibroin was grafted, The tensile strength of fibroin fiber artificial vascular material was improved, but the elasticity and hydrophilicity of the material decreased. The tensile strength of fibroin fiber artificial vascular materials was also improved after grafting with low molecular weight regenerated silk fibroin. The grafted fibroin fiber had better homogeneity and stability, and the degree of elasticity and hydrophilicity decreased slightly. The optimized graft concentration was 1% high molecular weight regenerated silk fibroin solution and 5% low molecular weight regenerated silk fibroin solution. (3) High molecular weight regenerated silk fibroin was grafted. Fibroin fiber artificial vascular materials adsorb more plasma albumin. The results of hemolysis and cytotoxicity test showed that compared with low molecular weight regenerated fibroin, the graft of high molecular weight regenerated fibroin could significantly improve the blood and cytocompatibility of the materials. To sum up, the surface morphology of silk fibroin fiber artificial vascular material can be kept intact by He plasma treatment, and the mechanical property loss is small, and the hydrophilicity is improved obviously. Compared with the low molecular weight regenerated fibroin protein, grafted high molecular weight regenerated fibroin protein can obviously improve the biocompatibility of fibroin fiber artificial vascular materials, but there are some problems such as the decrease of material elasticity after grafting. The grafting process needs to be further improved.
【学位授予单位】：东华大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R318.08
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